Department of Obstetrics, Yidu Central Hospital of Weifang, Weifang, Shandong, China (mainland).
Yidu Central Hospital of Weifang, Weifang, Shandong, China (mainland).
Med Sci Monit. 2018 Jun 21;24:4262-4270. doi: 10.12659/MSM.908766.
BACKGROUND The POZ/BTB and AT-hook-containing Zinc finger protein 1 (PATZ1) is a ubiquitously expressed transcription factor belonging to the POZ domain Krüppel-like zinc finger (POK) family. It is involved in the pathogenesis of a growing list of human diseases, including cancer. The effect of PATZ1 on serous ovarian carcinoma (SOC) remains unclear. This study initially explored the clinical significance of PATZ1 in patients with SOC, the relationship between its expression and the prognosis of SOC patients, and its role in tumor proliferation and invasion. MATERIAL AND METHODS Immunohistochemistry and quantitative real-time polymerase chain reaction (qPCR) were performed to characterize the expression of PATZ1 in SOC tissues. The relationship between PATZ1 expression and the clinicopathological features of patients with SOC was analyzed by chi-square test. Kaplan-Meier method and Cox regression analyses were utilized to evaluate the prognosis of SOC. PATZ1-constructed transfection-mediated overexpression was conducted. The CCK-8 assay was performed to examine the proliferation, while Transwell assay was used to detect the invasive capability. RESULTS The results of IHC and qPCR analyses showed that the expression of PATZ1 in cancerous tissue was significantly lower than that in non-cancerous tissues. Meanwhile, PATZ1 expression was significantly associated with tumor differentiation and LN metastasis. Survival analysis showed that PATZ1 expression was one of the independent prognosis factors for overall survival of SOC patients. In addition, overexpression of PATZ1 inhibited the proliferation and invasion of OVCAR3 cells by in vitro experiments. CONCLUSIONS Our data suggest that PATZ1 is a novel prognostic marker in SOC.
POZ/BTB 和 AT-hook 结构域锌指蛋白 1（PATZ1）是一种普遍表达的转录因子，属于 POZ 结构域 Krüppel 样锌指（POK）家族。它参与了越来越多的人类疾病的发病机制，包括癌症。PATZ1 对浆液性卵巢癌（SOC）的影响尚不清楚。本研究初步探讨了 PATZ1 在 SOC 患者中的临床意义、其表达与 SOC 患者预后的关系及其在肿瘤增殖和侵袭中的作用。
采用免疫组织化学和实时定量聚合酶链反应（qPCR）技术检测 SOC 组织中 PATZ1 的表达。采用卡方检验分析 PATZ1 表达与 SOC 患者临床病理特征的关系。采用 Kaplan-Meier 法和 Cox 回归分析评估 SOC 的预后。通过构建 PATZ1 转染介导过表达，CCK-8 检测增殖，Transwell 检测侵袭能力。
免疫组化和 qPCR 分析结果显示，癌组织中 PATZ1 的表达明显低于非癌组织。同时，PATZ1 表达与肿瘤分化和 LN 转移显著相关。生存分析显示，PATZ1 表达是 SOC 患者总生存的独立预后因素之一。此外，体外实验表明，过表达 PATZ1 可抑制 OVCAR3 细胞的增殖和侵袭。
我们的数据表明，PATZ1 是 SOC 的一种新的预后标志物。
