Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia (IIT@CRIB), Largo Barsanti e Matteucci, 53, I-80125, Napoli, Italy.
Biomater Sci. 2018 Jul 24;6(8):2084-2091. doi: 10.1039/c7bm01093a.
In tissue engineering there is growing interest in fabricating highly engineered platforms designed to instruct cells towards the synthesis of tissues that reproduce their natural counterpart. In this context, a fundamental factor to take into account is the control over the final tissue orientation, especially for what concerns the replication of load-bearing tissues whose functions are strictly related to their microstructural organization. Starting from this point, in this work we have engineered a gelatin-based hydrogel in order to be patterned by 2-photon polymerization (2PP) lithography for the fabrication of instructive free standing building blocks designed to produce anisotropic collagen-based μtissues. Biological results clearly highlighted the strong relationship between μtissue orientation and such topographies, which resulted in a crucial element in the production of highly anisotropic μtissues.
在组织工程领域，人们越来越感兴趣地制造高度工程化的平台，旨在指导细胞朝着合成能够复制其天然对应物的组织的方向发展。在这方面，需要考虑的一个基本因素是对最终组织方向的控制，特别是对于那些承载负荷的组织的复制，其功能与它们的微观结构组织密切相关。基于这一点，在这项工作中，我们设计了一种基于明胶的水凝胶，以便通过双光子聚合（2PP）光刻进行图案化，制造用于产生各向异性基于胶原蛋白的μ组织的指导性独立建筑块。生物学结果清楚地强调了μ组织方向与这些形貌之间的强相关性，这是产生高度各向异性μ组织的关键因素之一。
