Kanno T, Saito T, Saito A
Brain Res. 1985 Jul 15;338(2):237-42. doi: 10.1016/0006-8993(85)90152-0.
The redox states of cytochromes a(a3), b and c + c1 were continuously measured on the surface of the perfused bullfrog brain with the aid of a scanning spectrophotometer. To correlate with changes of the redox state, electrical activity was simultaneously recorded in the vicinity of the tip of the light guide of the spectrophotometer. In most cases, parallel reduction of the cytochromes was recorded in concordance with burst activity when the third or the fourth dorsal root of the spinal cord was electrically stimulated for 2 min. Recurrent cytochrome reduction and burst activity were produced by stimulation for a longer period, 5 or 10 min. Both the cytochrome reduction and burst activity were abolished when 1 microM tetrodotoxin was added to the perfusing solution. These results suggest that the cytochrome reduction coinciding with electrical activity in the brain may correlate with the Na+-influx followed by the activation of Na+,K+-adenosine triphosphatase in the neurons.
借助扫描分光光度计，在灌注的牛蛙脑表面连续测量细胞色素a（a3）、b以及c + c1的氧化还原状态。为了与氧化还原状态的变化相关联，在分光光度计光导尖端附近同时记录电活动。在大多数情况下，当脊髓第三或第四背根受到电刺激2分钟时，会记录到细胞色素的平行还原，与爆发活动一致。长时间（5或10分钟）刺激会产生反复的细胞色素还原和爆发活动。当向灌注溶液中添加1微摩尔河豚毒素时，细胞色素还原和爆发活动均被消除。这些结果表明，大脑中与电活动同时发生的细胞色素还原可能与神经元中Na +内流以及随后Na +，K + - 三磷酸腺苷酶的激活有关。
