Kanno T, Saito T
Brain Res. 1986 Mar 26;369(1-2):275-84. doi: 10.1016/0006-8993(86)90536-6.
Redox states of cytochromes a(a3), b and c+c1 were continuously measured on the surface of the cerebral hemisphere of the perfused bullfrog brain with the aid of a scanning organ spectrophotometer. To correlate with changes of the redox state, electrical activity of the brain was simultaneously recorded in the vicinity of the tip of the light guide of the spectrophotometer. Electrical stimulation of the third dorsal root of the spinal cord induced parallel reduction of the cytochromes and burst activity in the brain. Intracerebroventricular (i.c.v.) infusion of vasoactive intestinal polypeptide (VIP; 30 pmol/100 g body weight) nullified the cytochrome reduction induced by the electrical stimulation of the dorsal root, but did not attenuate the burst activity. The i.c.v. infusion of VIP (30 pmol/100 g b.wt.) per se had little if any influence upon both redox state and burst activity. The infusion of 1 nM VIP into the internal carotid arteries also nullified the cytochrome reduction response to the afferent electrical activation. The present results provide evidence that one of the physiological roles of the VIPergic neurons in the brain may be to balance the redox states of electron transfer systems in neurons.
借助扫描器官分光光度计，在灌流牛蛙脑的大脑半球表面连续测量细胞色素a（a3）、b以及c + c1的氧化还原状态。为了与氧化还原状态的变化相关联，在分光光度计光导尖端附近同时记录大脑的电活动。对脊髓第三背根进行电刺激会导致细胞色素平行还原以及大脑中的爆发活动。脑室内（i.c.v.）注入血管活性肠肽（VIP；30 pmol / 100 g体重）可消除由背根电刺激诱导的细胞色素还原，但不会减弱爆发活动。脑室内注入VIP（30 pmol / 100 g体重）本身对氧化还原状态和爆发活动几乎没有影响。向内颈动脉注入1 nM VIP也消除了对传入电激活的细胞色素还原反应。目前的结果提供了证据，表明脑中VIP能神经元的生理作用之一可能是平衡神经元中电子传递系统的氧化还原状态。
