全球范围内磷酸化蛋白质的鉴定
Identification of Phosphorylated Proteins on a Global Scale.
作者信息
Iliuk Anton
机构信息
Tymora Analytical Operations, Innovations, West Lafayette, Indiana.
出版信息
Curr Protoc Chem Biol. 2018 Sep;10(3):e48. doi: 10.1002/cpch.48. Epub 2018 May 31.
Abstract
Liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) has enabled researchers to analyze complex biological samples with unprecedented depth. It facilitates the identification and quantification of modifications within thousands of proteins in a single large-scale proteomic experiment. Analysis of phosphorylation, one of the most common and important post-translational modifications, has particularly benefited from such progress in the field. Here, detailed protocols are provided for a few well-regarded, common sample preparation methods for an effective phosphoproteomic experiment. © 2018 by John Wiley & Sons, Inc.
摘要
液相色谱（LC）与串联质谱（MS/MS）联用，使研究人员能够以前所未有的深度分析复杂的生物样品。它有助于在单个大规模蛋白质组学实验中对数千种蛋白质中的修饰进行鉴定和定量。磷酸化是最常见且重要的翻译后修饰之一，该领域的这一进展使其分析尤其受益。本文提供了一些备受认可的常见样品制备方法的详细方案，用于有效的磷酸化蛋白质组学实验。© 2018 约翰威立父子出版公司。
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