Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, China.
Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan, China.
PLoS One. 2018 Jun 21;13(6):e0199262. doi: 10.1371/journal.pone.0199262. eCollection 2018.
The NAM, ATAF1/2, and CUC2 (NAC) family constitutes a large family of plant-specific transcription factors, involved in many aspects of physiological processes and a variety of abiotic stresses. There is little information concerning the NAC family in Sesamum indicum. In this study, 87 sesame NAC genes were identified and phylogenetically clustered into 12 groups with Arabidopsis NAC genes. A total of 83 SiNAC genes were distributed non-randomly on the 16 linkage groups in sesame. Four and 49 SiNACs were found to be tandemly and segmentally duplicated, respectively. Expression profiles of SiNAC genes in different tissues (root, stem, leaf, flower, seed, and capsule) and in response to drought and waterlogging stresses by using RNA-seq data demonstrated that 23 genes were highly expressed in all tissues, 18 and 31 SiNACs respond strongly to drought and waterlogging stresses, respectively. In addition, the expression of 30 SiNAC genes distributed in different subgroups was analyzed with quantitative real-time RT-PCR under cold, osmotic, and salt stresses, revealed that their expression patterns vary in response to abiotic stresses. SiNAC genes displayed diverse expression patterns among the different tissues and stress treatments, suggested that their contribution to plant growth and development in sesame and multiple stress resistance in sesame. In this study, NAC transcription factors were analyzed in sesame and some specific candidate SiNAC genes in response to abiotic stress for functional study were identified. This study provides valuable information to deepen our understanding of the abiotic stress responses by NAC transcription factors in sesame.
NAM、ATAF1/2 和 CUC2（NAC）家族构成了一个庞大的植物特异性转录因子家族，参与许多生理过程和各种非生物胁迫。芝麻中关于 NAC 家族的信息很少。在这项研究中，鉴定了 87 个芝麻 NAC 基因，并与拟南芥 NAC 基因聚类为 12 组。总共 83 个 SiNAC 基因分布在芝麻的 16 个连锁群上，分布不均匀。发现 4 个和 49 个 SiNAC 分别为串联和片段重复。使用 RNA-seq 数据研究 SiNAC 基因在不同组织（根、茎、叶、花、种子和蒴果）中的表达谱以及对干旱和水淹胁迫的响应，表明 23 个基因在所有组织中高度表达，18 和 31 个 SiNAC 对干旱和水淹胁迫有强烈响应。此外，还通过定量实时 RT-PCR 分析了不同亚组中 30 个 SiNAC 基因在冷、渗透和盐胁迫下的表达，表明它们的表达模式因非生物胁迫而异。SiNAC 基因在不同组织和胁迫处理中的表达模式不同，表明它们在芝麻的生长发育和多种胁迫抗性中发挥作用。本研究分析了芝麻中的 NAC 转录因子，并鉴定了一些对非生物胁迫有反应的特定候选 SiNAC 基因进行功能研究。这项研究为深入了解 NAC 转录因子在芝麻中的非生物胁迫反应提供了有价值的信息。
