Hyde D R, Tu C P
Cell. 1985 Sep;42(2):629-38. doi: 10.1016/0092-8674(85)90120-5.
We have identified a new gene, tnpM, in Tn21 that encodes the 12.6 kilodalton modulator protein. The Tn21 modulator enhances Tn21 transposition and suppresses resolution of cointegrate replicons in vivo. A putative binding site may be located in the N-terminal portion of the TnpR (resolvase) structural gene sequences. Tn501 transposition and cointegrate resolution can be regulated by the subcloned tnpM gene of Tn21 in trans-complementation experiments. Examination of the Tn501 DNA sequence also reveals a potential tnpM coding sequence upstream of the Tn501 resolvase gene. We conclude that Tn21 and Tn501 are different from Tn3 and Tn1000 both in genome organization and in regulation of transposition functions.
我们在Tn21中鉴定出一个新基因tnpM，它编码12.6千道尔顿的调节蛋白。Tn21调节蛋白可增强Tn21转座，并在体内抑制共整合复制子的解离。一个推定的结合位点可能位于TnpR（解离酶）结构基因序列的N端部分。在反式互补实验中，Tn501的转座和共整合解离可由Tn21亚克隆的tnpM基因进行调控。对Tn501 DNA序列的检测还揭示了Tn501解离酶基因上游存在一个潜在的tnpM编码序列。我们得出结论，Tn21和Tn501在基因组组织和转座功能调控方面均与Tn3和Tn1000不同。
