Ploy M C, Denis F, Courvalin P, Lambert T
Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 87042 Limoges, France.
Antimicrob Agents Chemother. 2000 Oct;44(10):2684-8. doi: 10.1128/AAC.44.10.2684-2688.2000.
Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences of the amplification products. Sixteen clinical isolates had a class 1 integron, 2 contained an additional class 1 or class 2 integron, but no class 3 integron was detected. Thirteen strains had integrons with a single cassette: aac(3)-Ia (9 strains), ant(2")-Ia (2 strains), or aac(6')-Ib (2 strains); 1 had aac(6')-Ib and oxa20 cassettes and an unknown gene; and 1 had an integron containing ant(2")-Ia and an oxa3 cassette truncated by IS6100. The remaining strains harbored class 1 integrons with gene cassettes previously found in Enterobacteriaceae. One integron had a hybrid structure composed of intI2 and the 3' conserved segment of class 1 integrons. These data indicate that integrons play a major role in multidrug resistance in Acinetobacter.
对20株对多种抗生素耐药的鲍曼不动杆菌菌株进行整合子含量及扩增产物序列分析。16株临床分离株含有1类整合子，2株含有额外的1类或2类整合子，但未检测到3类整合子。13株菌株的整合子带有单个盒式结构：aac(3)-Ia（9株）、ant(2")-Ia（2株）或aac(6')-Ib（2株）；1株含有aac(6')-Ib和oxa20盒式结构及一个未知基因；1株的整合子含有ant(2")-Ia和一个被IS6100截断的oxa3盒式结构。其余菌株携带的1类整合子带有先前在肠杆菌科中发现的基因盒式结构。一个整合子具有由intI2和1类整合子的3'保守区段组成的杂合结构。这些数据表明整合子在鲍曼不动杆菌的多重耐药中起主要作用。
