Sharaf M A, Rooney D W
Cell Biol Int Rep. 1985 Jun;9(6):561-7. doi: 10.1016/0309-1651(85)90021-9.
Division in chemostat cultures of Chlamydomonas reinhardi was synchronized by daily cycles of both temperature and light. In response to 8 h cool (20 degrees C) - dim light/16 h warm (28 degrees C) - bright light cycles and continuous dilution that removed half the cells daily, the cells replicated nuclear DNA late in the warm-bright phase; released an average of two daughter zoospores early in the warm-bright phase of the next cycle. In these synchronized green algal cultures all ATP and most cAMP were intracellular; most cGMP (approximately 91%) was extracellular. Early in the warm-bright phase ATP doubled after which cAMP level fell 25%. During DNA replication cAMP level doubled, rising to a modest peak reached as replication ended.
莱茵衣藻恒化器培养中的细胞分裂通过温度和光照的每日循环实现同步。响应8小时低温（20摄氏度）-弱光/16小时高温（28摄氏度）-强光循环以及每天去除一半细胞的连续稀释，细胞在高温-强光阶段后期复制核DNA；在下一个循环的高温-强光阶段早期平均释放两个子游动孢子。在这些同步化的绿藻培养物中，所有ATP和大部分cAMP都在细胞内；大部分cGMP（约91%）在细胞外。在高温-强光阶段早期，ATP翻倍，之后cAMP水平下降25%。在DNA复制期间，cAMP水平翻倍，在复制结束时升至适度峰值。
