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微小RNA-196b通过靶向GATA6促进肺癌细胞的迁移和侵袭。

miR-196b promotes lung cancer cell migration and invasion through the targeting of GATA6.

作者信息

Li Hongli, Feng Chao, Shi Songtao

机构信息

Department of Operation Room, Eastern Medical District of Linyi People's Hospital, Linyi, Shandong 276034, P.R. China.

Department of Surgery, Eastern Medical District of Linyi People's Hospital, Linyi, Shandong 276034, P.R. China.

出版信息

Oncol Lett. 2018 Jul;16(1):247-252. doi: 10.3892/ol.2018.8671. Epub 2018 May 8.

DOI:10.3892/ol.2018.8671
PMID:29928408
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6006457/
Abstract

MicroRNAs (miRNAs) have been proven to regulate gene expression at the protein translation level. miRNA abnormal expression has been associated with the development of lung cancer. In the present study, we aimed to investigate the mechanism of miR-196 in non-small cell lung cancer (NSCLC). The miR-196b and GATA-6 (GATA6) expression levels were examined in NSCLC by RT-qPCR and western blot analysis. Transwell assay was used to assess cell migration and invasion. Moreover, the specific target of miR-196b in NSCLC was verified by the luciferase reporter assay. The expression of miR-196b was higher in both NSCLC tissues and cells than the normal levels. Specifically, the miR-196b mimic group in NSCLC cells markedly promoted cell migration and invasion, while the miR-196b inhibitor group exhibited the opposite effect. Furthermore, GATA6 was verified as a specific target of miR-196b in NSCLC cells and GATA6 could attenuate the migratory and invasive ability of NSCLC cells regulated by miR-196b. In addition, the relationship between GATA6 and miR-196b expression was negatively correlated in NSCLC tissues. Thus, miR-196b enhanced NSCLC cell migration and invasion via the downregulation of GATA6, indicating its potential application in NSCLC diagnosis and therapy.

摘要

微小RNA（miRNA）已被证明在蛋白质翻译水平上调节基因表达。miRNA异常表达与肺癌的发生发展有关。在本研究中，我们旨在探讨miR-196在非小细胞肺癌（NSCLC）中的作用机制。通过RT-qPCR和蛋白质印迹分析检测NSCLC中miR-196b和GATA-6（GATA6）的表达水平。采用Transwell实验评估细胞迁移和侵袭能力。此外，通过荧光素酶报告基因实验验证miR-196b在NSCLC中的特异性靶点。NSCLC组织和细胞中miR-196b的表达均高于正常水平。具体而言，NSCLC细胞中的miR-196b模拟物组显著促进细胞迁移和侵袭，而miR-196b抑制剂组则表现出相反的效果。此外，GATA6被证实为NSCLC细胞中miR-196b的特异性靶点，并且GATA6可以减弱miR-196b调节的NSCLC细胞的迁移和侵袭能力。此外，在NSCLC组织中，GATA6与miR-196b表达之间呈负相关。因此，miR-196b通过下调GATA6增强NSCLC细胞的迁移和侵袭能力，表明其在NSCLC诊断和治疗中的潜在应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/930584dae1e9/ol-16-01-0247-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/6f5f24872cf7/ol-16-01-0247-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/f7ae51bcedde/ol-16-01-0247-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/b5a686514557/ol-16-01-0247-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/930584dae1e9/ol-16-01-0247-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/6f5f24872cf7/ol-16-01-0247-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/f7ae51bcedde/ol-16-01-0247-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/b5a686514557/ol-16-01-0247-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d650/6006457/930584dae1e9/ol-16-01-0247-g03.jpg

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