Wang Linlin, Wang Xufei, Jiang Xuefeng
Ultrasound Department of China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
Department of Opthalmology, Jilin Province People's Hospital, Changchun, Jilin 130021, P.R. China.
Oncol Lett. 2019 Mar;17(3):3219-3226. doi: 10.3892/ol.2019.9955. Epub 2019 Jan 21.
Gastric cancer (GC) is a malignant tumor originating from the mucosal epithelium of the stomach. Patients suffering from this disease may have occurrence of residual GC due to delay in diagnosis and treatment. In addition, abnormal expression of microRNAs (miRNAs) is involved in GC progression. Therefore, we examined the underlying mechanism of miR-127 in GC. The expression of miR-127 and Wnt7a was examined in GC using RT-qPCR and western blot analysis. A Transwell assay was used to assess the ability of GC cell migration and invasion. Luciferase reporter assay was used to verify the specific target of miR-127 in GC. The results showed miR-127 expression was lower in GC than normal samples, while Wnt7a expression was detected at a higher level in GC than normal samples. The association between miR-127 and Wnt7a expression was negatively correlated in GC tissues. miR-127 mimic in the two GC cell lines markedly curbed cell migration and invasion, while inhibition of miR-127 showed the opposite effect. In addition, Wnt7a siRNA significantly inhibited GC cell migration and invasion and Wnt7a was verified as a specific target of miR-127 in GC cells. Wnt7a reversed the ability of GC cell migration and invasion regulated by miR-127. In conclusion, miR-127 could curb GC cell migration and invasion by upregulating Wnt7a, indicating its potential application in GC diagnosis and therapy.
胃癌（GC）是一种起源于胃黏膜上皮的恶性肿瘤。由于诊断和治疗延迟，患有这种疾病的患者可能会出现残留胃癌。此外，微小RNA（miRNA）的异常表达与胃癌进展有关。因此，我们研究了miR-127在胃癌中的潜在机制。使用RT-qPCR和蛋白质印迹分析检测胃癌中miR-127和Wnt7a的表达。采用Transwell试验评估胃癌细胞迁移和侵袭能力。荧光素酶报告基因试验用于验证miR-127在胃癌中的特异性靶点。结果显示，胃癌中miR-127的表达低于正常样本，而Wnt7a的表达在胃癌中高于正常样本。在胃癌组织中，miR-127与Wnt7a表达之间呈负相关。两种胃癌细胞系中的miR-127模拟物显著抑制细胞迁移和侵袭，而抑制miR-127则显示出相反的效果。此外，Wnt7a siRNA显著抑制胃癌细胞迁移和侵袭，并且Wnt7a被验证为胃癌细胞中miR-127的特异性靶点。Wnt7a逆转了miR-127调节的胃癌细胞迁移和侵袭能力。总之，miR-127可通过上调Wnt7a抑制胃癌细胞迁移和侵袭，表明其在胃癌诊断和治疗中的潜在应用价值。
