Lee Y C, Bacarese-Hamilton A J, Wood S M, Blumgart L H, Bloom S R
Clin Chim Acta. 1985 Jun 30;149(1):29-36. doi: 10.1016/0009-8981(85)90270-0.
To determine the nature of the neurotensin-like immunoreactivity produced by some cases of primary liver carcinoma, plasma and tumour neurotensin-like immunoreactivities were characterised in patients with neurotensin-producing hepatoma by chromatographic analysis in conjunction with radioimmunoassay with C- and N-terminally directed antisera. Gel filtration revealed that the majority of neurotensin-like immunoreactivity extracted from the tumour was co-eluted with synthetic neurotensin, whereas a substantial amount of the plasma immunoreactive neurotensin emerged at the void volume. Tumour neurotensin-like immunoreactivity showed a degree of heterogeneity on gel filtration and high pressure liquid chromatography that contrasted with neurotensin-like immunoreactivity extracted from ileal mucosa, suggesting a different post-translational processing of neurotensin within tumour tissue. The ratio of neurotensin 1-8 to neurotensin 1-13 in the patients' plasma suggested that neurotensin was metabolised in a manner similar to that in healthy subjects. The clinical and biological significance of the ectopic production of neurotensin in some hepatomas remains unclear.
为了确定某些原发性肝癌病例所产生的神经降压素样免疫反应性的性质，通过色谱分析结合使用针对C末端和N末端的抗血清的放射免疫测定，对产生神经降压素的肝癌患者的血浆和肿瘤神经降压素样免疫反应性进行了表征。凝胶过滤显示，从肿瘤中提取的大部分神经降压素样免疫反应性与合成神经降压素共洗脱，而大量的血浆免疫反应性神经降压素出现在空体积处。肿瘤神经降压素样免疫反应性在凝胶过滤和高压液相色谱上显示出一定程度的异质性，这与从回肠黏膜中提取的神经降压素样免疫反应性形成对比，表明肿瘤组织内神经降压素的翻译后加工不同。患者血浆中神经降压素1-8与神经降压素1-13的比例表明，神经降压素的代谢方式与健康受试者相似。某些肝癌中神经降压素异位产生的临床和生物学意义仍不清楚。
