Harper J, Schumacher B, Eisenstein R, Harper E
Curr Eye Res. 1985 Jul;4(7):803-6. doi: 10.3109/02713688509020037.
Bovine eyes were dissected and separate pools of lens, lens capsule, cornea and vitreous were extracted in guanidine, subjected to ultrafiltration, and examined for their effects on collagenolytic activity. Although lens extract was not inhibitory, the cornea and vitreous both contained inhibitors of collagenase. More inhibition was present in the filtrate of the vitreous than in the retentate, whereas the total amount of inhibition in the cornea was distributed almost equally between the two fractions. The inhibition observed was dose dependent. The partially purified inhibitors from cornea and vitreous blocked the activity of human skin and tadpole back skin collagenases, but they failed to inhibit the bacterial (Clostridium histolyticum) collagenase. The inhibitor was stable to heating to 60 degrees for 30 minutes and to trypsinization.
对牛眼进行解剖，分别提取晶状体、晶状体囊、角膜和玻璃体，置于胍中，进行超滤，并检测它们对胶原酶活性的影响。虽然晶状体提取物没有抑制作用，但角膜和玻璃体均含有胶原酶抑制剂。玻璃体滤液中的抑制作用比截留物中的更强，而角膜中的总抑制量在两个组分中几乎平均分布。观察到的抑制作用呈剂量依赖性。从角膜和玻璃体中部分纯化得到的抑制剂可阻断人皮肤和蝌蚪背部皮肤胶原酶的活性，但不能抑制细菌（溶组织梭菌）胶原酶的活性。该抑制剂在60℃加热30分钟及经胰蛋白酶处理后仍保持稳定。
