Department of Chemical Engineering, Jadavpur University, 188, Raja S. C. Mullick Road, Kolkata, 700 032, West Bengal, India.
Department of Environmental Science, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India.
J Environ Manage. 2018 Oct 1;223:185-195. doi: 10.1016/j.jenvman.2018.06.026. Epub 2018 Jun 19.
This study focuses on the investigation of removal of textile dye (Reactive Yellow) by a combined approach of sorption integrated with biodegradation using low cost adsorbent fly ash immobilized with Pseudomonas sp. To ensure immobilization of bacterial species on treated fly ash, fly ash with immobilized bacterial cells was characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and fluorescence microscopy. Comparative batch studies were carried out using Pseudomonas sp, fly ash and immobilized Pseudomonas sp on flyash and were observed that immobilized Pseudomonas sp on flyash acted as better decolourizing agent. The optimized pH, temperature, and immobilized adsorbent dosage for highest percentage of dye removal were observed to be pH 6, 303 K, 1.2 g/L in all the cases. At optimum condition, the highest percentage of dye removal was found to be 88.51%, 92.62% and 98.72% for sorption (flyash), biodegradation (Pseudomonas sp) and integral approach (Pseudomonas sp on flyash) respectively. Optimization of operating parameters of textile dye decolourization was done by response surface methodology (RSM) using Design Expert 7 software. Phytotoxicity evaluation with Cicer arietinum revealed that seeds exposed to untreated dye effluents showed considerably lower growth, inhibited biochemical, and enzyme parameters with compared to those exposed to treated textile effluents. Thus this immobilized inexpensive technique could be used for removal of synthetic dyes present in textile wastewater.
本研究采用吸附与生物降解相结合的方法，利用廉价吸附剂粉煤灰固定化假单胞菌，研究去除纺织染料（活性黄）。为了确保细菌物种在处理后的粉煤灰上固定化，使用傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）和荧光显微镜对固定有细菌细胞的粉煤灰进行了表征。使用假单胞菌、粉煤灰和固定在粉煤灰上的固定化假单胞菌进行了比较批处理研究，结果表明，固定在粉煤灰上的假单胞菌是更好的脱色剂。在所有情况下，观察到最佳 pH 值、温度和固定化吸附剂用量分别为 pH 6、303 K 和 1.2 g/L，以实现染料去除率最高。在最佳条件下，吸附（粉煤灰）、生物降解（假单胞菌）和综合方法（粉煤灰上的假单胞菌）的染料去除率最高分别为 88.51%、92.62%和 98.72%。通过使用 Design Expert 7 软件的响应面法（RSM）对纺织染料脱色的操作参数进行了优化。用鹰嘴豆进行的植物毒性评估表明，与暴露于处理后的纺织废水相比，暴露于未经处理的染料废水中的种子生长明显较低，生物化学和酶参数受到抑制。因此，这种固定化的廉价技术可用于去除纺织废水中存在的合成染料。
