Gasparotto Francielly Mourão, Lívero Francislaine Aparecida Dos Reis, Palozi Rhanany Allan Caloi, Ames Maria Leticia, Nunes Bruna, Donadel Guilherme, Ribeiro Rita de Cassia Lima, Lourenço Emerson Luiz Botelho, Kassuya Cândida Aparecida Leite, Junior Arquimedes Gasparotto
Laboratório de Eletrofisiologia e Farmacologia Cardiovascular, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
Laboratório de Pesquisa Pré-Clínica de Produtos Naturais, Universidade Paranaense, Umuarama, PR, Brazil.
Planta Med. 2018 Nov;84(17):1271-1279. doi: 10.1055/a-0644-2794. Epub 2018 Jun 21.
Excess weight and dyslipidemia are among the most serious health problems in Western societies. These conditions enhance the risk of cardiac disease and have been linked with a higher prevalence of cardiac arrhythmias and sudden death. The present study investigated the cardioprotective effects of on ventricular remodeling in rabbits that were fed a 1% cholesterol-rich diet. We first obtained an ethanol-soluble fraction of and performed a detailed phytochemical study by liquid chromatography-DAD/ESI-MS. For 60 days, male rabbits were fed the cholesterol-rich diet or a diet without the addition of cholesterol. After 30 days, different groups of rabbits were treated with the ethanol-soluble fraction of (10, 30, and 100 mg/kg, p. o.), simvastatin (2.5 mg/kg), or vehicle once daily for 30 days. At the end of 60 days, the serum lipoprotein ratio, electrocardiographic profile, histopathological alterations, and the cardiac antioxidant defense system were investigated. Echocardiographic analysis showed morphological and functional alterations in cholesterol-rich diet-fed animals, indicating left ventricle hypertrophy. The total cholesterol/high-density lipoprotein ratio and low-density lipoprotein/high-density lipoprotein ratio were significantly higher in cholesterol-rich diet-fed rabbits. Myocardial flaccidity, fatty degeneration, and concentric left ventricular hypertrophy were observed. An increase in lipid peroxidation levels, a decrease in superoxide dismutase activity, and a decrease in reduced glutathione levels were observed in the myocardium of all cholesterol-rich diet-fed rabbits. Treatment with the ethanol-soluble fraction of , especially the highest dose, significantly reduced all of these alterations, thus demonstrating the cardioprotective effect of the ethanol-soluble fraction of on cardiac changes that are induced by a cholesterol-rich diet.
超重和血脂异常是西方社会最严重的健康问题之一。这些情况会增加患心脏病的风险，并与心律失常和猝死的较高发生率有关。本研究调查了[具体物质名称未给出]对喂食1%富含胆固醇饮食的家兔心室重构的心脏保护作用。我们首先获得了[具体物质名称未给出]的乙醇可溶部分，并通过液相色谱 - DAD/ESI - MS进行了详细的植物化学研究。雄性家兔喂食富含胆固醇的饮食或不添加胆固醇的饮食，持续60天。30天后，不同组的家兔每天一次接受[具体物质名称未给出]的乙醇可溶部分（10、30和100mg/kg，口服）、辛伐他汀（2.5mg/kg）或赋形剂治疗，持续30天。在60天结束时，研究了血清脂蛋白比率、心电图特征、组织病理学改变和心脏抗氧化防御系统。超声心动图分析显示，喂食富含胆固醇饮食的动物存在形态和功能改变，表明左心室肥厚。喂食富含胆固醇饮食的家兔的总胆固醇/高密度脂蛋白比率和低密度脂蛋白/高密度脂蛋白比率显著更高。观察到心肌松弛、脂肪变性和同心性左心室肥厚。在所有喂食富含胆固醇饮食的家兔的心肌中，观察到脂质过氧化水平升高、超氧化物歧化酶活性降低和还原型谷胱甘肽水平降低。用[具体物质名称未给出]的乙醇可溶部分治疗，尤其是最高剂量，显著减少了所有这些改变，从而证明了[具体物质名称未给出]的乙醇可溶部分对富含胆固醇饮食诱导的心脏变化具有心脏保护作用。
