Ramesh E, Elanchezhian R, Sakthivel M, Jayakumar T, Senthil Kumar R S, Geraldine P, Thomas P A
Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Fundam Clin Pharmacol. 2008 Jun;22(3):275-84. doi: 10.1111/j.1472-8206.2008.00585.x.
Oxidative stress is believed to contribute to the pathogenesis of hypercholesterolaemic atherosclerosis; hence, various antioxidant compounds are being evaluated for potential anti-atherogenic effects. The present study assessed the efficacy of epigallocatechin gallate (EGCG), an antioxidant component of the plant Camellia sinensis, in improving serum lipid profile and antioxidant parameters in erythrocytes and cardiac tissue in rats fed an atherogenic diet. In male albino Wistar rats fed an atherogenic diet for 30 days, significantly increased serum levels of total cholesterol, triglycerides and lipoprotein cholesterol fractions and cardiac risk ratio were noted, compared with levels in rats fed a normal diet. Intraperitoneal administration of EGCG (100 mg/kg) for 7 or 15 days to the atherogenic diet-fed rats resulted in significantly lower serum levels of total cholesterol, triglycerides, low-density and very low density lipoprotein cholesterol fractions and a significantly higher serum level of high-density lipoprotein cholesterol compared with levels in atherogenic diet-fed, saline-treated rats. Significantly higher mean malondialdehyde levels and significantly lower mean activities of antioxidant enzymes and mean levels of non-enzymatic antioxidants occurred in atherogenic diet-fed rats compared with those fed a normal diet. When atherogenic diet-fed rats received EGCG treatment for 7 or 15 days, significantly lower mean levels of MDA, higher mean levels of non-enzymatic antioxidants and higher mean activities of enzymatic antioxidants occurred, compared with those in saline-treated rats. Thus, EGCG appears to ameliorate disruptions of serum lipid profile and of antioxidant parameters in erythrocyte and cardiac tissue of Wistar rats fed an atherogenic diet; these results may be relevant to treating human atherosclerosis.
氧化应激被认为与高胆固醇血症性动脉粥样硬化的发病机制有关；因此，正在评估各种抗氧化化合物的潜在抗动脉粥样硬化作用。本研究评估了表没食子儿茶素没食子酸酯（EGCG），一种茶树的抗氧化成分，对喂食致动脉粥样硬化饮食的大鼠血清脂质谱以及红细胞和心脏组织抗氧化参数的改善效果。与喂食正常饮食的大鼠相比，喂食致动脉粥样硬化饮食30天的雄性白化Wistar大鼠血清总胆固醇、甘油三酯和脂蛋白胆固醇组分水平以及心脏风险比显著升高。对喂食致动脉粥样硬化饮食的大鼠腹腔注射EGCG（100 mg/kg）7天或15天，与喂食致动脉粥样硬化饮食并接受生理盐水处理的大鼠相比，血清总胆固醇、甘油三酯、低密度和极低密度脂蛋白胆固醇组分水平显著降低，血清高密度脂蛋白胆固醇水平显著升高。与喂食正常饮食的大鼠相比，喂食致动脉粥样硬化饮食的大鼠丙二醛平均水平显著升高，抗氧化酶平均活性和非酶抗氧化剂平均水平显著降低。当喂食致动脉粥样硬化饮食的大鼠接受EGCG处理7天或15天时，与接受生理盐水处理的大鼠相比，丙二醛平均水平显著降低，非酶抗氧化剂平均水平升高，酶抗氧化剂平均活性升高。因此，EGCG似乎可以改善喂食致动脉粥样硬化饮食的Wistar大鼠的血清脂质谱以及红细胞和心脏组织抗氧化参数的紊乱；这些结果可能与治疗人类动脉粥样硬化有关。
