Simoes-Silva Rodrigo, Alfonso Jorge, Gomez Ana, Holanda Rudson J, Sobrinho Juliana C, Zaqueo Kayena D, Moreira-Dill Leandro S, Kayano Anderson M, Grabner Fernando P, da Silva Saulo L, Almeida Jose R, Stabeli Rodrigo G, Zuliani Juliana P, Soares Andreimar M
Centro de Estudos de Biomoleculas Aplicadas a Saude, CEBio, Fiocruz Rondonia, FIOCRUZ e Universidade Federal de Rondonia, UNIR, Porto Velho-RO, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia de Rondônia, IFRO, Brazil.
Curr Pharm Biotechnol. 2018;19(4):308-335. doi: 10.2174/1389201019666180620111025.
Research involving snake venom has gradually surpassed the simple discovery of new molecules using purification and structural characterization processes, and extended to the identification of their molecular targets and the evaluation of their therapeutic potential. Nevertheless, this only became possible due to constant progress in experimental biology and protein purification approaches.
This review aims to discuss the main components of snake venoms that have been investigated for biotechnological purposes, and to discover how these promising biomolecules were obtained with the satisfactory degree of purity that have enabled such studies. Advances in purification technologies of various snake venom molecules have allowed for important discoveries of proteins and peptides with different biomedical and biotechnological applications.
It is believed that significant experimental and computational advances will arise in similar proportions in the coming years that will allow researchers to map the molecular regions responsible for their pharmacological actions, their respective mechanisms of action and their cell targets.
涉及蛇毒的研究已逐渐超越了单纯利用纯化和结构表征过程发现新分子的阶段，扩展到了对其分子靶点的鉴定以及治疗潜力的评估。然而，这一切之所以成为可能，得益于实验生物学和蛋白质纯化方法的不断进步。
本综述旨在探讨已针对生物技术目的进行研究的蛇毒主要成分，并探究这些有前景的生物分子是如何以令人满意的纯度获得的，从而得以开展此类研究。各种蛇毒分子纯化技术的进步使得发现具有不同生物医学和生物技术应用的蛋白质和肽成为可能。
相信在未来几年，类似程度的重大实验和计算进展将会出现，这将使研究人员能够描绘出负责其药理作用、各自作用机制及其细胞靶点的分子区域。
