Liss Joanna, Pastuszek Ewa, Pukszta Sebastian, Hoffmann Eva, Kuczynski Waldemar, Lukaszuk Aron, Lukaszuk Krzysztof
INVICTA Fertility and Reproductive Center, ul. Trzy Lipy 3, 80-172 Gdansk, Poland.
Center for Chromosome Stability, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark.
Reprod Fertil Dev. 2018 Nov;30(12):1720-1727. doi: 10.1071/RD17428.
The present study analysed live birth ratios in frozen embryo transfer (FET) cycles where embryo ploidy status was determined with preimplantation genetic testing (PGT) using next-generation sequencing (NGS). PGT was performed on trophectoderm cells biopsied at the blastocyst stage. The present prospective cohort study included 112 women undergoing frozen embryo transfer, with NGS PGT. The control group consisted of 85 patients who underwent the IVF procedure with FET planned for a subsequent cycle. The live birth rate per cycle was higher by ~18.5 percentage points in the investigated compared with control group (42.0% vs 23.5% respectively; P=0.012). The differences between the study and control groups were also significant for clinical pregnancy (42.0% vs 23.5% respectively; P=0.012), implantation (41.2% vs 22.2% respectively; P=0.001) and pregnancy loss rates (9.6% vs 28.6% respectively; P=0.027). The results show that PGT NGS is a useful method for embryo selection and it may be implemented in routine clinical practice with propitious results.
本研究分析了冷冻胚胎移植（FET）周期中的活产率，在这些周期中，使用下一代测序（NGS）通过植入前基因检测（PGT）来确定胚胎的倍性状态。PGT在囊胚期活检的滋养外胚层细胞上进行。本前瞻性队列研究纳入了112例行冷冻胚胎移植并接受NGS PGT的女性。对照组由85例计划在下一周期进行FET的IVF患者组成。与对照组相比，研究组每个周期的活产率高出约18.5个百分点（分别为42.0%和23.5%；P=0.012）。研究组和对照组在临床妊娠（分别为42.0%和23.5%；P=0.012）、着床（分别为41.2%和22.2%；P=0.001）和流产率（分别为9.6%和28.6%；P=0.027）方面的差异也具有统计学意义。结果表明，PGT NGS是一种有用的胚胎选择方法，可在常规临床实践中应用并取得良好效果。
