Department of Immunology, School of Basic Medical Sciences, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Peking University Health Science Center, Beijing, China.
FEBS Lett. 2018 Jul;592(14):2444-2457. doi: 10.1002/1873-3468.13166. Epub 2018 Jul 5.
Parainfluenza virus infection is a common respiratory illness in children. Although lncRNAs are novel regulators of virus-induced innate immunity, a systemic attempt to characterize the differential expression of lncRNAs upon parainfluenza virus infection is lacking. In this report, we identify 207 lncRNAs and 166 mRNAs differentially expressed in SeV-infected HEK293T cells by microarray. The functional annotation analysis reveals that differentially regulated transcripts are predominantly involved in the host antiviral response pathway. The lncRNAs with the potential to regulate SeV-induced antiviral response are identified by building the lncRNA-mRNA coexpression network. Furthermore, silencing lncRNA ENST00000565297 results in reduced type I IFN signaling upon SeV infection. These catalogs may facilitate future analysis of the functions of lncRNAs in innate immunity and related diseases.
副流感病毒感染是儿童常见的呼吸道疾病。尽管长链非编码 RNA（lncRNA）是病毒诱导固有免疫的新型调节因子，但缺乏对副流感病毒感染时 lncRNA 差异表达的系统研究。在本报告中，我们通过微阵列鉴定了 SeV 感染的 HEK293T 细胞中 207 个 lncRNA 和 166 个 mRNA 的差异表达。功能注释分析表明，差异调控的转录本主要参与宿主抗病毒反应途径。通过构建 lncRNA-mRNA 共表达网络，鉴定出具有调节 SeV 诱导抗病毒反应潜力的 lncRNA。此外，沉默 lncRNA ENST00000565297 会导致 SeV 感染时 I 型 IFN 信号减弱。这些目录可能有助于进一步分析 lncRNA 在固有免疫和相关疾病中的功能。
