Tu Rong-Fang, Zhang Xiu-Feng, He Zhen-Hua
Department of Respiratory Medicine, the Second Affiliated Hospital of University of South China, Hengyang 421001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2016 Apr 8;32(4):365-369. doi: 10.13459/j.cnki.cjap.2016.04.020.
To observe the expression of 5-Hydroxytryptamine 2B receptor (5-HTR2B)、E-cadherin (E-cad)、alpha-smooth muscle actin(α-SMA) in the bleomycin -induced pulmonary fibrosis tissue of rats.
Forty-five healthy male SD rats were randomly di-vided into control group,bleomycin group and bleomycin+prednisone group(=15). Five rats in each group randomly sacrificed at the 7、the 14 and the 28 day after eastablishing models. The lung tissue was observed by microscope in HE and Masson staining. Lung hydroxypro-line (HYP) content was evaluated. The expression of protein and mRNA of 5-HTR2B, E-cad and α-SMA were analyzed by immunohistoche-mistry and/or RT-PCR.
A dynamic changes from alveolitis to pulmonary fibrosis could be observed in the slices by HE and Masson staining. The experimental results by immunohistochemistry and RT-PCR showed that the protein and mRNA expression of 5-HTR2B and a-SMA enhanced rat pulmonary fibrosis (<0.05) and reached the highest at the 28 day; the corresponding protein and mRNA expression of E-cad reduced (<0.05), and reduced to the lowest value at the 28 day. Compared with bleomycin group, the corresponding mRNA and protein expression of 5-HTR2B and a-SMA in bleomycin+prednisone group had decreased (<0.05); however, the corresponding mRNA and protein expression of E-cad had increased(<0.05).
5-HTR2B is involved in the pathogenesis of pulmonary fibrosis throught epithelial-mesenchymal transition (EMT).
观察5-羟色胺2B受体（5-HTR2B）、E-钙黏蛋白（E-cad）、α-平滑肌肌动蛋白（α-SMA）在博来霉素诱导的大鼠肺纤维化组织中的表达。
将45只健康雄性SD大鼠随机分为对照组、博来霉素组和博来霉素+泼尼松组（每组15只）。每组分别于造模后7天、14天和28天随机处死5只大鼠。取肺组织进行HE和Masson染色，显微镜下观察。检测肺组织羟脯氨酸（HYP）含量。采用免疫组织化学和/或RT-PCR分析5-HTR2B、E-cad和α-SMA的蛋白及mRNA表达。
HE和Masson染色切片显示从肺泡炎到肺纤维化的动态变化。免疫组织化学和RT-PCR实验结果显示，5-HTR2B和α-SMA的蛋白及mRNA表达在大鼠肺纤维化时增强（P<0.05），并在28天时达到最高；E-cad的相应蛋白及mRNA表达降低（P<0.05），并在28天时降至最低值。与博来霉素组相比，博来霉素+泼尼松组5-HTR2B和α-SMA的相应mRNA和蛋白表达降低（P<0.05）；然而，E-cad的相应mRNA和蛋白表达增加（P<0.05）。
5-HTR2B通过上皮-间质转化（EMT）参与肺纤维化的发病机制。
