Feng Shui-Dong, Wu Di, Yang Si-Si, He Jian-Qin, Zhang Kai-Fang, Ling Hong-Yan
Department of Social Medicine and Health Service Management, School of Public Health, Hengyang 421001, China.
Department of Physiology, School of Medicine, University of South China, Hengyang 421001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2016 Apr 8;32(4):370-372. doi: 10.13459/j.cnki.cjap.2016.04.021.
To observe the effects of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells and to ex-plore its possible mechanism.
Human breast cancer MCF-7 cells were treated with arecoline at the concentrations of 0,10,30,50, 100,300,500mol/L, the cell proliferation were detected by MTT assay, cell apoptosis were analyzed by Hoechst 33342 staining and flow cy-tometry, the protein expression of Bax,Bcl-2 and P53 were detected by Western blot.
Low concentration(0,10,30, 50 mol/L) arecoline had no effect on the proliferation and apoptosis of MCF-7. However, high concentration(100,300,500mol/L) arecoline inhibited proliferation and induced apoptosis of MCF-7 cells in a concentration-dependent manner, arecoline also significantly increased P53 and Bax protein expression and decreased Bcl-2 protein expression.
High concentration arecoline inhibited the proliferation and induced the apoptosis of MCF-7 cells, the mechanism was probably corrected with increasing P53 and Bax protein expression and decreasing Bcl-2 pro-tein expression.
观察槟榔碱对MCF-7人乳腺癌细胞增殖和凋亡的影响，并探讨其可能机制。
将人乳腺癌MCF-7细胞分别用浓度为0、10、30、50、100、300、500μmol/L的槟榔碱处理，采用MTT法检测细胞增殖情况，用Hoechst 33342染色和流式细胞术分析细胞凋亡情况，用蛋白质免疫印迹法检测Bax、Bcl-2和P53蛋白表达。
低浓度（0、10、30、50μmol/L）槟榔碱对MCF-7细胞的增殖和凋亡无影响。然而，高浓度（100、300、500μmol/L）槟榔碱以浓度依赖方式抑制MCF-7细胞增殖并诱导其凋亡，槟榔碱还显著增加P53和Bax蛋白表达，降低Bcl-2蛋白表达。
高浓度槟榔碱抑制MCF-7细胞增殖并诱导其凋亡，其机制可能与增加P53和Bax蛋白表达、降低Bcl-2蛋白表达有关。
