School of Nursing, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Department of Pharmacy, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
J Cell Biochem. 2018 Nov;119(10):8317-8324. doi: 10.1002/jcb.26876. Epub 2018 Jun 22.
We intended to evaluate miR-490-5p expression in hepatocellular carcinoma (HCC) tissues and detect the potential targets of miR-490-5p. In vitro experiments were conducted to further investigate the biological function of miR-490-5p on HCC cell metastasis. We investigated the abnormally expressed miRNAs in HCC tissues, and the miR-490-5p expression level was detected by qRT-PCR. E2F2 and ECT2 were proved to be the potential targets of miR-490-5p by luciferase reporter assay. The expression levels of E2F2 and ECT2 were determined using Western blot. Transwell assay was used to analyse the impact of miR-490-5p on metastasis of HCC cells. Four high-expressed miRNAs, and seven low-expressed miRNAs, including miR-490-5p, were detected in HCC tissues. The expression level of miR-490-5p was connected with the tumor size, tumor node metastasis (TNM) stage, and survival ratio of HCC patients. E2F2 and ECT2 were the targets of miR-490-5p, and miR-490-5p inhibited HCC cell metastasis through down-regulating the expressions of E2F2 and ECT2. The over-expressed miR-490-5p could restrain the metastasis of HCC cells by down-regulating E2F2 and ECT2 expression levels.
我们旨在评估肝癌 (HCC) 组织中 miR-490-5p 的表达，并检测 miR-490-5p 的潜在靶标。进行了体外实验以进一步研究 miR-490-5p 对 HCC 细胞转移的生物学功能。我们研究了 HCC 组织中异常表达的 miRNAs，并通过 qRT-PCR 检测 miR-490-5p 的表达水平。通过荧光素酶报告基因检测证实 E2F2 和 ECT2 是 miR-490-5p 的潜在靶标。通过 Western blot 测定 E2F2 和 ECT2 的表达水平。使用 Transwell 分析 miR-490-5p 对 HCC 细胞转移的影响。在 HCC 组织中检测到四种高表达的 miRNAs 和七种低表达的 miRNAs，包括 miR-490-5p。miR-490-5p 的表达水平与 HCC 患者的肿瘤大小、肿瘤淋巴结转移 (TNM) 分期和生存率有关。E2F2 和 ECT2 是 miR-490-5p 的靶标，miR-490-5p 通过下调 E2F2 和 ECT2 的表达抑制 HCC 细胞转移。过表达的 miR-490-5p 通过下调 E2F2 和 ECT2 的表达水平抑制 HCC 细胞的转移。
