Foster T J, Brown N L
J Bacteriol. 1985 Sep;163(3):1153-7. doi: 10.1128/jb.163.3.1153-1157.1985.
Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were oriented in the opposite direction to and divergently from the merTCAD genes. This shows that the reading frame previously thought to be merR was incorrect. Expression of the gene fusion was repressed in trans by a compatible plasmid carrying the R100 merR+ gene, as was a similarly oriented transcriptional fusion. In contrast, expression of beta-galactosidase by the lac fragment located at the same site but in the opposite orientation was at a lower level and was not repressed by merR+.
在携带源自质粒R100的mer基因的pBR322质粒中体外构建了R100 merR基因与lacZ之间的转录（操纵子）融合和翻译（基因）融合。翻译融合的方向与merTCAD基因相反且方向不同。这表明先前认为是merR的阅读框是错误的。携带R100 merR⁺基因的相容质粒可反式抑制基因融合的表达，同样方向的转录融合也是如此。相比之下，位于同一位点但方向相反的lac片段表达的β-半乳糖苷酶水平较低，且不受merR⁺的抑制。
