Mizuno Y, Yamazaki M, Takasawa T, Kizaki T, Shiokawa H
J Biochem. 1985 Apr;97(4):1135-42. doi: 10.1093/oxfordjournals.jbchem.a135158.
The amino acid sequence of acylphosphatase from porcine skeletal muscle was determined. It consists of 98 amino acid residues with N-acetylserine at the amino (N)-terminus: Ac-Ser-Thr-Ala-Arg-Pro-Leu-Lys-Ser-Val-Asp-Tyr-Glu-Val-Phe-Gly -Arg-Val-Gln-Gly-Val-Cys-Phe-Arg-Met-Tyr-Thr-Glu-Asp-Glu-Ala-Arg-Lys-Ile -Gly-Val-Val-Gly-Trp-Val-Lys-Asn-Thr-Ser-Lys-Gly-Thr-Val-Thr-Gly-Gln -Val-Gln-Gly-Pro-Glu-Glu-Lys-Val-Asn-Ser-Met-Lys-Ser-Trp-Leu-Ser-Lys -Ile-Gly-Ser-Pro-Ser-Ser-Arg-Ile-Asp-Arg-Thr-Asn-Phe-Ser-Asn-Glu-Lys- Thr-Ile-Ser-Lys-Leu-Glu-Tyr-Ser-Asn-Phe-Ser-Ile-Arg-Tyr-OH. This sequence has three substitutions of amino acid residues, i.e., Thr/Ala, Ile/Val, and Ile/Val at positions 26, 68, and 96, respectively, from that of horse muscle acylphosphatase, formerly the only mammalian acylphosphatase with known sequence.
测定了猪骨骼肌酰基磷酸酶的氨基酸序列。它由98个氨基酸残基组成，氨基（N）末端为N - 乙酰丝氨酸：Ac - Ser - Thr - Ala - Arg - Pro - Leu - Lys - Ser - Val - Asp - Tyr - Glu - Val - Phe - Gly - Arg - Val - Gln - Gly - Val - Cys - Phe - Arg - Met - Tyr - Thr - Glu - Asp - Glu - Ala - Arg - Lys - Ile - Gly - Val - Val - Gly - Trp - Val - Lys - Asn - Thr - Ser - Lys - Gly - Thr - Val - Thr - Gly - Gln - Val - Gln - Gly - Pro - Glu - Glu - Lys - Val - Asn - Ser - Met - Lys - Ser - Trp - Leu - Ser - Lys - Ile - Gly - Ser - Pro - Ser - Ser - Arg - Ile - Asp - Arg - Thr - Asn - Phe - Ser - Asn - Glu - Lys - Thr - Ile - Ser - Lys - Leu - Glu - Tyr - Ser - Asn - Phe - Ser - Ile - Arg - Tyr - OH。该序列与马肌肉酰基磷酸酶（以前唯一已知序列的哺乳动物酰基磷酸酶）相比，分别在第26、68和96位有三个氨基酸残基的替换，即Thr/Ala、Ile/Val和Ile/Val。
