Barra D, Schinina M E, Simmaco M, Bannister J V, Bannister W H, Rotilio G, Bossa F
J Biol Chem. 1984 Oct 25;259(20):12595-601.
The complete amino acid sequence of manganese superoxide dismutase from human liver was determined. The sequence was deduced following characterization of the peptides obtained from tryptic, chymotryptic, and Staphylococcus aureus digests of the apoprotein. Chemical cleavage with dimethyl sulfoxide-hydrobromic acid was also carried out. The amino acid sequence listed below is made up of 196 amino acids and the two subunit polypeptides in the native enzyme appear to be identical. No homology was observed with copper/zinc containing class of superoxide dismutase. Lys-His-Ser-Leu-Pro-Asp-Leu-Pro-Tyr-Asp-Tyr-Gly-Ala-Leu-Glu-Pro-His-Il e -Asn-Ala-Gln-Ile-Met-Gln-Leu-His-His-Ser-Lys-His-His-Ala-Ala-Tyr-Val-Asn -Asn-Leu-Asn-Val-Thr-Gln-Glu-Lys-Tyr-Gln-Glu-Ala-Leu-Ala-Lys-Gly-Asp-Val -Thr-Ala-Gln-Ile-Ala-Leu-Gln-Pro-Ala-Leu-Lys-Phe-Asn-Gly-Gly-Gly-His-Ile -Asn-His-Ser-Ile-Phe-Trp-Thr-Asn-Leu-Ser-Pro-Asn-Gly-Gly-Gly-Gln-Pro-Lys -Gly-Glu-Leu-Leu-Glu-Ala-Ile-Lys-Arg-Asp-Phe-Gly-Ser-Phe-Asp-Lys-Phe-Lys -Gln-Lys-Leu-Thr-Ala-Ala-Ser-Val-Gly-Val-Gln-Gly-Ser-Gly-Trp-Leu-Gly-Phe -Asn-Lys-Gln-Arg-Gly-His-Leu-Gln-Ile-Ala-Ala-Cys-Pro-Asn-Gln-Asp-Pro-Leu -Gln-Gly-Thr-Thr-Gly-Leu-Ile-Pro-Leu-Leu-Gly-Ile-Asp-Val-Trp-Glu-His-Ala -Tyr-Tyr-Leu-Gln-Tyr-Lys-Asn-Val-Arg-Pro-Asp-Tyr-Leu-Lys-Ala-Ile-Trp-Asn -Val-Ile-Asn-Trp-Glu-Asn-Val-Thr-Glu-Arg-Tyr-Met-Ala-Cys-Lys-Lys.
测定了人肝脏中锰超氧化物歧化酶的完整氨基酸序列。该序列是在对脱辅基蛋白经胰蛋白酶、胰凝乳蛋白酶和金黄色葡萄球菌消化后得到的肽段进行表征后推导出来的。还进行了二甲基亚砜 - 氢溴酸化学裂解。下面列出的氨基酸序列由196个氨基酸组成，天然酶中的两个亚基多肽似乎是相同的。未观察到与含铜/锌类超氧化物歧化酶有同源性。赖 - 组 - 丝 - 亮 - 脯 - 天冬 - 亮 - 脯 - 酪 - 天冬 - 酪 - 甘 - 丙 - 亮 - 谷 - 脯 - 组 - 异亮 - 天冬 - 丙 - 谷氨酰胺 - 异亮 - 甲硫 - 谷氨酰胺 - 亮 - 组 - 组 - 丝 - 赖 - 组 - 组 - 丙 - 丙 - 酪 - 缬 - 天冬 - 天冬 - 亮 - 天冬 - 缬 - 苏 - 谷氨酰胺 - 谷 - 赖 - 酪 - 谷氨酰胺 - 谷 - 丙 - 亮 - 丙 - 赖 - 甘 - 天冬 - 缬 - 苏 - 丙 - 谷氨酰胺 - 异亮 - 丙 - 亮 - 谷氨酰胺 - 脯 - 丙 - 亮 - 赖 - 苯丙 - 天冬 - 甘 - 甘 - 甘 - 组 - 异亮 - 天冬 - 组 - 丝 - 异亮 - 苯丙 - 色 - 苏 - 天冬 - 亮 - 丝 - 脯 - 天冬 - 甘 - 甘 - 甘 - 谷氨酰胺 - 脯 - 赖 - 甘 - 谷 - 亮 - 亮 - 谷 - 丙 - 异亮 - 赖 - 精 - 天冬 - 苯丙 - 甘 - 丝 - 苯丙 - 天冬 - 赖 - 苯丙 - 赖 - 谷氨酰胺 - 赖 - 亮 - 苏 - 丙 - 丙 - 丝 - 缬 - 甘 - 缬 - 谷氨酰胺 - 甘 - 丝 - 甘 - 色 - 亮 - 甘 - 苯丙 - 天冬 - 赖 - 谷氨酰胺 - 精 - 甘 - 组 - 亮 - 谷氨酰胺 - 异亮 - 丙 - 丙 - 半胱 - 脯 - 天冬 - 谷氨酰胺 - 天冬 - 脯 - 亮 - 谷氨酰胺 - 甘 - 苏 - 苏 - 甘 - 亮 - 异亮 - 脯 - 亮 - 亮 - 甘 - 异亮 - 天冬 - 缬 - 色 - 谷 - 组 - 丙 - 酪 - 酪 - 亮 - 谷氨酰胺 - 酪 - 赖 - 天冬 - 缬 - 精 - 脯 - 天冬 - 酪 - 亮 - 赖 - 丙 - 异亮 - 色 - 天冬 - 缬 - 异亮 - 天冬 - 色 - 谷 - 天冬 - 缬 - 苏 - 谷 - 精 - 酪 - 甲硫 - 丙 - 半胱 - 赖 - 赖
