与大鼠核糖体蛋白S26信使核糖核酸互补的DNA的分子克隆及核苷酸序列

Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA.

作者信息

Kuwano Y, Nakanishi O, Nabeshima Y, Tanaka T, Ogata K

出版信息

J Biochem. 1985 Apr;97(4):983-92. doi: 10.1093/oxfordjournals.jbchem.a135175.

DOI:10.1093/oxfordjournals.jbchem.a135175

Abstract

A cDNA clone specific for rat ribosomal protein S26 was isolated by a positive hybridization translation assay from a cDNA library made for 8-9S poly(A)mRNA from regenerating rat liver. The nucleotide sequence of the cDNA was determined. The sequence contains 23 base pairs in the 5' noncoding region, 345 base pairs in the protein coding region and 67 base pairs in the 3' noncoding region besides the poly(A) tail. The primary structure of the protein S26 was deduced from the nucleotide sequence. It consists of 115 amino acids. Its molecular weight is 13,015 and its pI is about 11.1. The calculated amino acid composition is consistent with the reported composition of S26. From the results of Southern blot analysis, the protein S26 appears to have multiple genes.

摘要

通过阳性杂交翻译分析，从为再生大鼠肝脏的8 - 9S多聚腺苷酸（poly(A)）mRNA构建的cDNA文库中分离出了一个对大鼠核糖体蛋白S26特异的cDNA克隆。测定了该cDNA的核苷酸序列。该序列在5'非编码区含有23个碱基对，在蛋白质编码区含有345个碱基对，在3'非编码区除了多聚(A)尾外含有67个碱基对。从核苷酸序列推导了蛋白S26的一级结构。它由115个氨基酸组成。其分子量为13,015，其等电点约为11.1。计算得到的氨基酸组成与报道的S26组成一致。从Southern印迹分析结果来看，蛋白S26似乎有多个基因。