Tanaka T, Kuwano Y, Ishikawa K, Ogata K
Department of Biochemistry, Yamagata University School of Medicine, Japan.
Eur J Biochem. 1988 Apr 5;173(1):53-6. doi: 10.1111/j.1432-1033.1988.tb13965.x.
We constructed cDNA libraries from 8-9-S poly(A)-rich RNA from regenerating rat liver, isolated clones specific for ribosomal protein L27 and determined the nucleotide sequences of the cDNA clones. The longest cDNA consists of 15 base pairs from the 5' leading sequence, the entire coding sequence of 411 base pairs, and the 3' trailing sequence of 59 base pairs including the poly(A) tail. The primary structure of protein L27 was deduced from the sequence of nucleotides. Protein L27 contains 135 amino acids and has a molecular mass of 15,666 Da. The amino-terminal sequence agreed well with the published partial amino acid sequence and the calculated amino acid composition is also consistent with the reported composition determined for the hydrolyzate of L27.
我们从再生大鼠肝脏富含8 - 9 - S多聚腺苷酸的RNA构建了cDNA文库，分离出核糖体蛋白L27特异性的克隆，并测定了这些cDNA克隆的核苷酸序列。最长的cDNA由5'前导序列的15个碱基对、411个碱基对的完整编码序列以及包括多聚腺苷酸尾在内的59个碱基对的3'尾随序列组成。从核苷酸序列推导了蛋白L27的一级结构。蛋白L27含有135个氨基酸，分子量为15,666道尔顿。氨基末端序列与已发表的部分氨基酸序列吻合良好，计算出的氨基酸组成也与报道的L27水解产物的组成一致。
