Piwnica-Worms H, Keene J D
J Biol Chem. 1985 Sep 5;260(19):10503-11.
Permissive infections of BHK cells and nonpermissive infections of Raji cells were probed for the accumulation of vesicular stomatitis virus intracellular RNAs. In Raji cells, the onset of vesicular stomatitis virus transcription and replication was delayed when compared to BHK cells, and the accumulation of plus and minus sense leader RNAs was significantly reduced. In contrast, full length plus and minus strand replicative RNAs accumulated in Raji cells to levels approximately equivalent to those in BHK cells. In both cell types, approximately four times as many minus strands as plus strands were detected late in the infections. At 16 h postinfection, 12% of the total genomic RNA synthesized in BHK cells was packaged and released whereas only 0.8% was released from Raji cells. In addition, of those particles released by Raji cells, only 1% were infectious whereas 77% of those released by BHK cells were infectious. The virions released from both cell types contained similar amounts of the five viral proteins, however. Analysis of virions from Raji cells revealed a faster electrophoretic mobility of the glycoprotein than the glycoprotein in virions released from BHK cells. These results suggest that Raji cells may be restricted in their ability to support a complete infection at the level of virus assembly rather than at the level of RNA replication.
研究了水泡性口炎病毒在BHK细胞中的允许性感染以及在Raji细胞中的非允许性感染，以检测细胞内RNA的积累情况。与BHK细胞相比，Raji细胞中水泡性口炎病毒转录和复制的起始延迟，正链和负链前导RNA的积累显著减少。相比之下，全长正链和负链复制性RNA在Raji细胞中的积累水平与BHK细胞中的大致相当。在两种细胞类型中，感染后期检测到的负链数量约为正链的四倍。感染后16小时，BHK细胞中合成的总基因组RNA中有12%被包装并释放，而Raji细胞中只有0.8%被释放。此外，Raji细胞释放的颗粒中只有1%具有感染性，而BHK细胞释放的颗粒中有77%具有感染性。然而，两种细胞类型释放的病毒粒子所含的五种病毒蛋白量相似。对Raji细胞释放的病毒粒子分析显示，其糖蛋白的电泳迁移率比BHK细胞释放的病毒粒子中的糖蛋白更快。这些结果表明，Raji细胞在支持完整感染方面的能力可能在病毒组装水平而非RNA复制水平受到限制。
