Leppert M, Kolakofsky D
J Virol. 1980 Sep;35(3):704-9. doi: 10.1128/JVI.35.3.704-709.1980.
Vesicular stomatitis virus-infected cells contain short RNA transcripts, called leader RNAs, which are coded by the exact 3' end of both the minus-strand and plus-strand nucleocapsid templates. The molar amounts of both the plus-strand leader RNA (which is templated from the minus-strand genome) and the minus-strand leader RNA (which is templated from the plus-strand antigenome) were determined both in standard-virus- and mixed-virus-infected cells by using end-labeled genome probes. The results demonstrate that the presence of defective interfering particles in the infecting virus stock decreases the amount of plus-strand leader RNA but increases the amount of minus-strand leader RNA found in the infected cells. In addition, considerably more minus-strand leader RNA per mole of nucleocapsid template is synthesized in mixed-virus-infected cells than plus-strand leader RNA per mole of nucleocapsid template in both standard-virus- and mixed-virus-infected cells.
水泡性口炎病毒感染的细胞含有短RNA转录本，称为前导RNA，它们由负链和正链核衣壳模板的精确3'端编码。通过使用末端标记的基因组探针，在标准病毒感染和混合病毒感染的细胞中测定了正链前导RNA（以负链基因组为模板）和负链前导RNA（以正链反基因组为模板）的摩尔量。结果表明，感染病毒悬液中存在缺陷干扰颗粒会减少感染细胞中正链前导RNA的量，但会增加负链前导RNA的量。此外，在混合病毒感染的细胞中，每摩尔核衣壳模板合成的负链前导RNA比标准病毒感染和混合病毒感染的细胞中每摩尔核衣壳模板合成的正链前导RNA要多得多。
