Schubert D, LaCorbiere M
J Cell Biol. 1985 Sep;101(3):1071-7. doi: 10.1083/jcb.101.3.1071.
Adherons are high molecular weight glycoprotein complexes which are released into the growth medium of cultured cells. They mediate the adhesive interactions of many cell types, including those of embryonic chick neural retina. The cell surface receptor for chick neural retina adherons has been purified, and shown to be a heparan sulfate proteoglycan (Schubert, D., and M. LaCorbiere, 1985, J. Cell Biol., 100:56-63). This paper describes the isolation and characterization of a protein in neural retina adherons which interacts specifically with the cell surface receptor. The 20,000-mol-wt protein, called retinal purpurin (RP), stimulates neural retina cell-substratum adhesion and prolongs the survival of neural retina cells in culture. The RP protein interacts with heparin and heparan sulfate, but not with other glycosaminoglycans. Monovalent antibodies against RP inhibit RP-cell adhesion as well as adheron-cell interactions. The RP protein is found in neural retina, but not in other tissues such as brain and muscle. These data suggest that RP plays a role in both the survival and adhesive interactions of neural retina cells.
黏附素是一种高分子量糖蛋白复合物，会释放到培养细胞的生长培养基中。它们介导多种细胞类型的黏附相互作用，包括胚胎鸡神经视网膜细胞。鸡神经视网膜黏附素的细胞表面受体已被纯化，并证明是一种硫酸乙酰肝素蛋白聚糖（舒伯特，D.，和M.拉科比埃，1985年，《细胞生物学杂志》，100:56 - 63）。本文描述了神经视网膜黏附素中一种与细胞表面受体特异性相互作用的蛋白质的分离和特性。这种分子量为20,000的蛋白质，称为视网膜紫红素（RP），可刺激神经视网膜细胞与底物的黏附，并延长培养的神经视网膜细胞的存活时间。RP蛋白与肝素和硫酸乙酰肝素相互作用，但不与其他糖胺聚糖相互作用。针对RP的单价抗体可抑制RP与细胞的黏附以及黏附素与细胞的相互作用。RP蛋白存在于神经视网膜中，但不存在于脑和肌肉等其他组织中。这些数据表明，RP在神经视网膜细胞的存活和黏附相互作用中均发挥作用。
