使用随机引物法构建传染性支气管炎病毒（一种大型RNA病毒）的cDNA文库。
The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus.
作者信息
Binns M M, Boursnell M E, Foulds I J, Brown T D
出版信息
J Virol Methods. 1985 Jul;11(3):265-9. doi: 10.1016/0166-0934(85)90116-8.
Abstract
An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.
摘要
以传染性支气管炎病毒为例，描述了一种从大型RNA病毒生成基因文库的有效方法。已对随机引物克隆进行了表征，发现它们代表包括5'前导序列在内的病毒基因组。
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