禽传染性支气管炎病毒的mRNA A上存在一个前导序列。

A leader sequence is present on mRNA A of avian infectious bronchitis virus.

作者信息

Brown T D, Boursnell M E, Binns M M

出版信息

J Gen Virol. 1984 Aug;65 ( Pt 8):1437-42. doi: 10.1099/0022-1317-65-8-1437.

Abstract

The subgenomic mRNAs of the fowl coronavirus infectious bronchitis virus (IBV) form a 3' co-terminal or 'nested' set. The RNase T1 oligonucleotide mapping data which demonstrated this form of sequence organization provided no evidence for the presence of non-contiguous (leader) sequences fused to the 5' termini of the mRNAs. However, we have been able to demonstrate, by extension of an end-labelled synthetic oligonucleotide primer, the presence of a leader sequence on IBV mRNA A. Sequencing by the chemical degradation method of the extended product has yielded the almost complete sequence of the 60 base leader and its point of fusion to the sequence of the virus genomic RNA.

摘要

禽冠状病毒传染性支气管炎病毒（IBV）的亚基因组mRNA形成一个3' 共末端或“嵌套”集合。证明这种序列组织形式的核糖核酸酶T1寡核苷酸图谱数据数据未数据没有提供证据表明存在与mRNA 5' 末端融合的非连续（前导）序列。然而，我们能够通过延伸末端标记的合成寡核苷酸引物，证明IBV mRNA A上存在前导序列。通过化学降解法对延伸产物进行测序，已得到60个碱基的前导序列的几乎完整序列及其与病毒基因组RNA序列的融合点。