Kietz Christa, Pollari Vilma, Meinander Annika
Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland.
Curr Protoc Toxicol. 2018 Aug;77(1):e52. doi: 10.1002/cptx.52. Epub 2018 Jun 22.
As several diseases have been linked to dysbiosis of the human intestinal microflora, manipulation of the microbiota has emerged as an exciting new strategy for potentially treating and preventing diseases. However, the human microbiota consists of a plethora of different species, and distinguishing the impact of a specific bacterial species on human health is challenging. In tackling this challenge, the fruit fly Drosophila melanogaster, with its far simpler microbial composition, has emerged as a powerful model for unraveling host-microbe interactions. To study the interplay between the resident commensal microbiome and the host, flies can be made germ-free, or axenic. To elucidate the impact of specific bacteria, axenic flies can then be re-introduced to specific microbial species. In this unit, we provide a step-by-step protocol on how to rear Drosophila melanogaster under axenic conditions and confirm the axenity of flies. © 2018 by John Wiley & Sons, Inc.
由于多种疾病已被证明与人类肠道微生物群失调有关，因此对微生物群进行调控已成为一种令人兴奋的全新策略，有望用于治疗和预防疾病。然而，人类微生物群由大量不同物种组成，区分特定细菌物种对人类健康的影响具有挑战性。为应对这一挑战，具有简单得多的微生物组成的果蝇已成为揭示宿主-微生物相互作用的强大模型。为了研究常驻共生微生物群与宿主之间的相互作用，可以使果蝇无菌或无特定病原体。为了阐明特定细菌的影响，然后可以将无菌果蝇重新引入特定的微生物物种。在本单元中，我们提供了一份关于如何在无菌条件下饲养黑腹果蝇并确认果蝇无菌状态的分步方案。© 2018约翰威立国际出版公司。
