Hu Haiyan, Wang Yiyu, Zhang Yihui, Wang Wenhua, Xu Dongmei, Chen Zhiyu, Zhang Xiaoyan, Mao Dandan
J Tradit Chin Med. 2016 Oct;36(5):654-62. doi: 10.1016/s0254-6272(16)30086-3.
To investigate the effects of Qingxinkaiqiao (QK) compound in a rat model of Alzheimer's disease induced with β-amyloid (Aβ) 1-40.
Fifty-six three months, male, Sprague-Dawley rats were randomly divided into seven groups: blank control group, surgery group, model group, low-dose QK group, middle-dose QK group, high-dose QK group, and Aricept (donepezil hydrochloride) group, with eight rats in each group. Apart from the control and surgery groups, an Alzheimer's disease model was established in all groups by bilateral hippocampal injection of Aβ 1-40. The surgery group received an injection of the same volume of physiological saline. Two days after model establishment, rats from the drug groups were administered the corresponding drugs; the control group and model group were administered an equal volume of physiological saline for 14 days. After treatment, real-time quantitative polymerase chain reaction, immunohistochemistry, and western blot assay were employed to confirm mRNA and protein expressions of Bcl-2, Bax, caspase-3, and Aβ, respectively.
QK treatment resulted in significantly up-regulated Bcl-2 expression, down-regulated Bax, caspase-3, and Aβ expression, and reduced numbers of apoptotic cells in the cortex.
研究清心开窍（QK）复方对β-淀粉样蛋白（Aβ）1-40诱导的阿尔茨海默病大鼠模型的影响。
将56只3个月大的雄性Sprague-Dawley大鼠随机分为7组：空白对照组、假手术组、模型组、低剂量QK组、中剂量QK组、高剂量QK组和安理申（盐酸多奈哌齐）组，每组8只。除对照组和假手术组外，其余各组均通过双侧海马注射Aβ 1-40建立阿尔茨海默病模型。假手术组注射等量生理盐水。造模后2天，给药组大鼠给予相应药物；对照组和模型组给予等量生理盐水，持续14天。治疗后，分别采用实时定量聚合酶链反应、免疫组织化学和蛋白质印迹法检测Bcl-2、Bax、半胱天冬酶-3和Aβ的mRNA和蛋白表达。
QK治疗可显著上调Bcl-2表达，下调Bax、半胱天冬酶-3和Aβ表达，并减少皮质中凋亡细胞的数量。
