Hyo-Seok Seo, Hyun Jae Lee, Choong Jae Lee
J Tradit Chin Med. 2016 Oct;36(5):663-70. doi: 10.1016/s0254-6272(16)30087-5.
To investigate the effect of Pyunkanghwan (Pyunkang-tang) extract (PGT) on secretion of airway mucin in an experimental animal model involving hyperplasia of goblet cells and mucus hypersecretion, and to test its effects on bleomycin (BLM)-induced pulmonary fibrosis in vivo.
The protective activity of orally administered PGT was assessed in two rat pulmonary disease models. Effects on hypersecretion of pulmonary mucin in sulfur dioxide (SO2)-induced bronchitis in rats were assessed by quantifying the amount of mucus secreted and examining histopathology in the tracheal epithelium. In a rat model for BLMinduced pulmonary fibrosis, toxicity to the pulmonary system was examined by measuring levels of malondialdehyde and hydroxyproline, indicators of lipid peroxides and collagen, respectively, in lung tissue 28 days post-BLM treatment. Serial sections of lung tissue were stained with Masson trichrome to visualize collagen deposition. Effects of PGT on collagen synthesis were also assessed in vitro, in a cell culture model.
PGT inhibited mucin secretion and normalized SO2-induced increased muco- substances in goblet cells. In the BLM- induced model, PGT decreased the characteristic histopathological features of lung fibrosis and inhibited fibrotic lesions, as indicated by decreased hydroxyproline content. PGT also inhibited the BLM-induced increase in malondialdehyde levels, demonstrating its protective effect against lipid peroxidation in cell membranes of the lung. In MLg 2908 mouse lung fibroblast cells, PGT decreased transforming growth factor (TGF)-β-stimulated type I collagen synthesis.
PGT can inhibit both hypersecretion of airway mucins and pulmonary fibrosis.
在一个涉及杯状细胞增生和黏液分泌过多的实验动物模型中，研究平康丸（平康汤）提取物（PGT）对气道黏液分泌的影响，并在体内测试其对博来霉素（BLM）诱导的肺纤维化的作用。
在两种大鼠肺部疾病模型中评估口服PGT的保护活性。通过量化分泌的黏液量并检查气管上皮的组织病理学，评估对二氧化硫（SO₂）诱导的大鼠支气管炎中肺黏液分泌过多的影响。在BLM诱导的肺纤维化大鼠模型中，在BLM治疗后28天，通过测量肺组织中丙二醛和羟脯氨酸的水平来检查对肺系统的毒性，丙二醛和羟脯氨酸分别是脂质过氧化物和胶原蛋白的指标。肺组织连续切片用Masson三色染色以观察胶原蛋白沉积。还在细胞培养模型中体外评估PGT对胶原蛋白合成的影响。
PGT抑制黏液分泌，并使SO₂诱导的杯状细胞中黏液物质增加恢复正常。在BLM诱导的模型中，PGT减轻了肺纤维化的特征性组织病理学特征并抑制了纤维化病变，羟脯氨酸含量降低表明了这一点。PGT还抑制了BLM诱导的丙二醛水平升高，表明其对肺细胞膜脂质过氧化具有保护作用。在MLg 2908小鼠肺成纤维细胞中，PGT减少了转化生长因子（TGF）-β刺激的I型胶原蛋白合成。
PGT可以抑制气道黏液分泌过多和肺纤维化。
