Zhang Si-Jie, Qian Zheng, Liu Jing-Jing, Zhang Xin-Feng, Si Jin-Ping
State Key Laboratory Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(10):2025-2031. doi: 10.19540/j.cnki.cjcmm.20180307.009.
The study is aimed to fully analyze antioxidant activty and stability of color-related components from Dendrobium officinale flower. The contents of anthocyanins,carotenoids and flavonoids of 10 varieties including whole flower and labellum of D.officinale flower were detected using UV visible spectroscopy technique and evaluated for their 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·), superoxide anion radical (·O⁻₂) and hydroxy radical (·OH) scavenging activty. And the effects of 4 kinds of metal ions (Cu²⁺, Mg²⁺, Al³⁺,K⁺) and 2 kinds of food additives were investigated on the stability of anthocyanins pigment from D.officinale flower. The results indicated that the flower of D.officinale contained anthocyanins,carotenoids and flavonoids,and their contents in different parts of different families varied. The content of anthocyanins (1 330.9 μg·g⁻¹) in labellum was significantly higher than that in the whole flower (696.3 μg·g⁻¹). The content of carotenoids was positively correlated with the content of anthocyanins,and there was significant positive correlation between the scavenging capacities of the three free radicals and the contents of anthocyanin,respectively (0.415,0.384,0.455). The Cu²⁺, Mg²⁺, Al³⁺, K⁺ and glucose showed significant color-enhancing effect and could increase the stability of anthocyanin in D.officinale flower,while ascorbic acids showed remarkably degradation effect of anthocyanin in D.officinale flower.
本研究旨在全面分析铁皮石斛花中与颜色相关成分的抗氧化活性及稳定性。采用紫外可见光谱技术检测了包括铁皮石斛花整花和唇瓣在内的10个品种的花色苷、类胡萝卜素和黄酮类化合物的含量,并对其清除1,1-二苯基-2-苦基肼自由基(DPPH·)、超氧阴离子自由基(·O⁻₂)和羟基自由基(·OH)的活性进行了评价。同时考察了4种金属离子(Cu²⁺、Mg²⁺、Al³⁺、K⁺)和2种食品添加剂对铁皮石斛花中花色苷色素稳定性的影响。结果表明,铁皮石斛花中含有花色苷、类胡萝卜素和黄酮类化合物,不同品种不同部位的含量存在差异。唇瓣中花色苷含量(1 330.9 μg·g⁻¹)显著高于整花(696.3 μg·g⁻¹)。类胡萝卜素含量与花色苷含量呈正相关,三种自由基的清除能力与花色苷含量之间分别存在显著正相关(0.415、0.384、0.455)。Cu²⁺、Mg²⁺、Al³⁺、K⁺和葡萄糖对铁皮石斛花中花色苷有显著增色作用,可提高其稳定性,而抗坏血酸对铁皮石斛花中花色苷有显著降解作用。