[Comparative studies on scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale].

OBJECTIVE

To study the scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale.

METHOD

The types and contents of flavonoids from different parts of D. officinale were analyzed by TLC and HPLC. The antioxidant effect was tested by scavenging DPPH free radicals activity.

RESULT

The stems, leaves and flowers contained the same type of flavone C-A glycosides and 8 common peaks were identified. The content of flavone C-A glycosides was significantly different. The content of flavone C-glycosides in leaves and flowers was higher than that in stems. The flavonoid in roots was less. Stems contained naringenin, which was not identified in root, leave and flower. Both stems and leaves had antioxidant capacity of eliminating DPPH free radicals, of which scavenging DPPH free radicals activity of leaves was better than stems.

CONCLUSION

Considering the content of flavonoid and antioxidant activity leave and flower of D. officinale may substitute stems. The study provides a preliminary basis for the development and utilization of leave and flower of D. officinale.