Wei Wei, Zhou Xiao-Mao, Wang Yu-Cheng, Lin Zhi-Cheng, Xue Ji-Hua
Affiliated Rehabilitation Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China.
Institute of Fujian Rehabilitation Industry, Fuzhou 350003, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(10):2147-2152. doi: 10.19540/j.cnki.cjcmm.20180104.025.
Alisol A 24-acetate, a triterpenoid extracted from Alisma orientale, has shown anti-atherosclerotic actions and many studies have proved that oxidized low density lipoprotein (Ox-LDL) could promote proliferation of aorta smooth muscle cells (VSMCs) which are closely related to atherosclerosis (AS). The purpose of this study was to evaluate the effect of alisol A 24-acetate on the proliferation of VSMCs isolated from the thoracic aorta of rats induced by ox-LDL. VSMCs were induced by ox-LDL(50 mg·L⁻¹) to establish the proliferation model and intervened by alisol A 24-acetate (5, 10, 20 mg·L⁻¹) for 12, 24 and 48 h. Then the proliferation of VSMCs was detected by MTT assay; protein expression levels of VSMCs PCNA, cyclinD1, cyclinE, p21, p27 and VSMCs PCNA, p21and p27 mRNA expression levels were detected by Western blot and Real-time polymerase chain reaction (RT-PCR) respectively. The results showed that ox-LDL could induce the proliferation of VSMCs (<0.05), increase the protein expression levels of PCNA, cyclinD1 and cyclinE in the VSMCs (<0.05) and inhibit the protein and mRNA expression levels of p21 and p27 (<0.05). As compared with the model group, alisol A 24-acetate inhibited the proliferation of VSMCs in rats induced by ox-LDL and inhibited the protein expression of VSMCs PCNA, cyclinD1, cyclinE and enhanced the protein and mRNA p21 and p27 expression levels (<0.05). The effect was more obvious with the increase of concentration of alisol A 24-acetate. These data indicate that alisol A 24-acetate can inhibit the proliferation of VSMCs induced by ox-LDL and the mechanism may be associated with inhibiting expression of cyclin protein, including cyclinD1, cyclinE, p21, p27 and so on.
泽泻醇A 24 - 乙酸酯是从东方泽泻中提取的一种三萜类化合物，已显示出抗动脉粥样硬化作用，许多研究证明氧化低密度脂蛋白（Ox-LDL）可促进与动脉粥样硬化（AS）密切相关的主动脉平滑肌细胞（VSMCs）增殖。本研究旨在评估泽泻醇A 24 - 乙酸酯对ox-LDL诱导的大鼠胸主动脉分离的VSMCs增殖的影响。用ox-LDL（50 mg·L⁻¹）诱导VSMCs建立增殖模型，并用泽泻醇A 24 - 乙酸酯（5、10、20 mg·L⁻¹）干预12、24和48小时。然后通过MTT法检测VSMCs的增殖；分别通过蛋白质印迹法和实时聚合酶链反应（RT-PCR）检测VSMCs增殖细胞核抗原（PCNA）、细胞周期蛋白D1、细胞周期蛋白E、p21、p27的蛋白表达水平以及VSMCs中PCNA、p21和p27的mRNA表达水平。结果表明，ox-LDL可诱导VSMCs增殖（<0.05），增加VSMCs中PCNA、细胞周期蛋白D1和细胞周期蛋白E的蛋白表达水平（<0.05），并抑制p21和p27的蛋白及mRNA表达水平（<0.05）。与模型组相比，泽泻醇A 24 - 乙酸酯抑制ox-LDL诱导的大鼠VSMCs增殖，抑制VSMCs中PCNA、细胞周期蛋白D1、细胞周期蛋白E的蛋白表达，并增强p21和p27的蛋白及mRNA表达水平（<0.05）。随着泽泻醇A 24 - 乙酸酯浓度的增加，作用更明显。这些数据表明，泽泻醇A 24 - 乙酸酯可抑制ox-LDL诱导的VSMCs增殖，其机制可能与抑制细胞周期蛋白包括细胞周期蛋白D1、细胞周期蛋白E、p21、p27等的表达有关。
