Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris, F-75005, France.
Institut de Biologie et Chimie des Protéines, CNRS UMR5086, Université de Lyon 1, Lyon, France.
Sci Rep. 2018 Jun 22;8(1):9509. doi: 10.1038/s41598-018-27826-y.
The exon junction complex (EJC) deposited on spliced mRNAs, plays a central role in the post-transcriptional gene regulation and specific gene expression. The EJC core complex is associated with multiple peripheral factors involved in various post-splicing events. Here, using recombinant complex reconstitution and transcriptome-wide analysis, we showed that the EJC peripheral protein complexes ASAP and PSAP form distinct complexes with the EJC core and can confer to EJCs distinct alternative splicing regulatory activities. This study provides the first evidence that different EJCs can have distinct functions, illuminating EJC-dependent gene regulation.
外显子衔接复合体（EJC）沉积在拼接的 mRNA 上，在转录后基因调控和特定基因表达中发挥核心作用。EJC 核心复合物与多个参与各种剪接后事件的外围因子相关。在这里，我们使用重组复合物重建和转录组全分析表明，EJC 外围蛋白复合物 ASAP 和 PSAP 与 EJC 核心形成不同的复合物，并赋予 EJC 不同的可变剪接调节活性。这项研究首次提供了不同的 EJC 可以具有不同功能的证据，阐明了 EJC 依赖性基因调控。
