Zhao Xueyan, Chu Qing, Cui Junxia, Xu Tianjun
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, 201306, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China.
Dev Comp Immunol. 2018 Oct;87:171-175. doi: 10.1016/j.dci.2018.06.009. Epub 2018 Jun 20.
Pattern recognition receptors can recognize pathogens, and then cells are induced to produce pro-inflammatory cytokines and interferon by multiple signaling pathways. Nevertheless, excessive inflammation disrupts immune homeostasis, thereby inducing autoimmune and inflammatory diseases. Thus, the regulation of immune responses is extremely important for host to keep homeostasis. In this study, we found that miR-19a plays a negative regulator in MyD88-mediated NF-κB signaling pathway by targeting MyD88 in miiuy croaker. Furthermore, over-expression of miR-19a in macrophages suppresses the expression of MyD88 and its downstream signaling genes of IRAK1, IRAK4 and TRAF6, whereas, the inhibitor of miR-19a has opposite effect. This study can increase our knowledge and help us to furthermore understand miRNAs regulatory mechanism in teleost fish.
模式识别受体能够识别病原体，随后细胞通过多种信号通路被诱导产生促炎细胞因子和干扰素。然而，过度炎症会破坏免疫稳态，从而诱发自身免疫性疾病和炎症性疾病。因此，免疫反应的调节对于宿主维持稳态极为重要。在本研究中，我们发现miR-19a通过靶向鮸鱼中的MyD88在MyD88介导的NF-κB信号通路中发挥负调控作用。此外，巨噬细胞中miR-19a的过表达抑制了MyD88及其下游信号基因IRAK1、IRAK4和TRAF6的表达，而miR-19a的抑制剂则具有相反的作用。本研究能够增加我们的知识，并帮助我们进一步了解硬骨鱼中miRNA的调控机制。
