Chang Renjie, Zheng Weiwei, Sun Yuena, Xu Tianjun
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, 201306, China.
Dev Comp Immunol. 2021 Jun;119:104025. doi: 10.1016/j.dci.2021.104025. Epub 2021 Feb 1.
Innate immune response is an important response mechanism for the host to achieve self-protection, and it plays an important role in identifying pathogens and resisting pathogen invasion. Growing evidences have shown that microRNA functions as a crucial regulator involved in the host innate immune response. In this study, the regulations of miR-1388-5p to regulate NF-κB signaling pathways via targeting the IRAK1 gene was studied in miiuy croaker. First, through bioinformatics software prediction, we found that IRAK1 is the direct target of miR-1388-5p, and then the prediction results were verified by using dual-luciferase assays. Next, we found that both miR-1388-5p mimics and pre-miR-1388 plasmids inhibit IRAK1 expression by complementing the seed sequence in the 3'-untranslated region (3'-UTR) of IRAK1. Finally, we observed that miR-1388-5p could negatively regulate NF-κB pathways through targeting IRAK1. These results provide new insights into the function of miR-1388-5p in fish innate immunity, meanwhile enriching miRNA-mediated regulatory networks.
天然免疫反应是宿主实现自我保护的重要反应机制，在识别病原体和抵抗病原体入侵中发挥重要作用。越来越多的证据表明，微小RNA作为关键调节因子参与宿主天然免疫反应。本研究在大黄鱼中研究了miR-1388-5p通过靶向IRAK1基因调控NF-κB信号通路的机制。首先，通过生物信息学软件预测，我们发现IRAK1是miR-1388-5p的直接靶标，然后利用双荧光素酶报告基因检测验证了预测结果。接下来，我们发现miR-1388-5p模拟物和pre-miR-1388质粒均通过与IRAK1 3'-非翻译区（3'-UTR）中的种子序列互补来抑制IRAK1表达。最后，我们观察到miR-1388-5p可通过靶向IRAK1对NF-κB通路进行负调控。这些结果为miR-1388-5p在鱼类天然免疫中的功能提供了新见解，同时丰富了miRNA介导的调控网络。
