School of Chemical Science & Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
School of Chemical Science & Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
J Proteomics. 2018 Jul 30;184:34-38. doi: 10.1016/j.jprot.2018.06.007. Epub 2018 Jun 20.
Core histones and their PTMs play important roles in regulating gene transcription and other DNA-related processes. The study of core histones PTMs, their cross-talk and functional roles is not only of broad biological significance but also of wide pathological and clinical relevance. Having the strength of comprehensive proteoform identification with 100% amino acid sequence coverage and combinatorial PTMs, top-down proteomics has become the state-of-the-art analytical tool for combinatorial PTM characterization of core histones. In this study, we report our top-down characterization of chicken (Gallus gallus domesticus) core histones, which have been widely used as models for chromosome re-construction among others because of easy availability and not-so-dense PTMs. With nanoRPLC-MS/MS analysis and ProteinGoggle database search, a total of 58 proteoforms were identified for the core histone families of H4, H2B, H2A, and H3.
核心组蛋白及其 PTMs 在调节基因转录和其他与 DNA 相关的过程中发挥着重要作用。研究核心组蛋白 PTMs、它们的串扰和功能作用不仅具有广泛的生物学意义，而且具有广泛的病理学和临床相关性。具有综合的蛋白质形式鉴定能力，可实现 100%的氨基酸序列覆盖和组合 PTMs，自上而下的蛋白质组学已成为核心组蛋白组合 PTM 特征描述的最先进的分析工具。在这项研究中，我们报告了对鸡（Gallus gallus domesticus）核心组蛋白的自上而下的表征，由于其易于获得和 PTMs 不密集等特点，鸡核心组蛋白被广泛用作染色体重建等模型。通过纳升级反相高效液相色谱-串联质谱分析和 ProteinGoggle 数据库搜索，共鉴定了 H4、H2B、H2A 和 H3 核心组蛋白家族的 58 种蛋白质形式。
