Departamento de Veterinária, Universidade Federal de Viçosa, Centro, Av. P.H. Rolfs s/n, Viçosa, MG, 36570-000, Brazil.
Centro Nacional de Pesquisa e Conservação de Mamíferos Carnívoros, Estrada Municipal Hisaichi Takebayashi, 8600, Bairro da Usina, Atibaia, SP, 12.952-011, Brazil.
Anim Reprod Sci. 2018 Aug;195:1-7. doi: 10.1016/j.anireprosci.2017.12.019. Epub 2017 Dec 26.
This study was designed to evaluate the efficiency of medetomidine anesthesia for semen collection through urethral catheter in wild and captive jaguars. Six captive and five wild jaguars were chemically restrained with a combination of medetomidine (0.08-0.1 mg/kg) and ketamine (5 mg/kg). After medetomidine administration the urethra was catheterized using a urinary tomcat catheter (1 mm diameter × 130 mm length) to collect semen from all animals. By using this technique, we could collect an average of 347.2 μl of semen containing 2,635.2 sperm/ml. Forward progressive motility, sperm progressive motility, and sperm morphology analysis demonstrated that the methodology did not affect sperm quality. Thus, urethral catheterization after medetomidine administration is a practical and efficient method to collect high-quality semen from wild and captive jaguars; this will enable the development of reproductive assisted technologies for jaguars.
本研究旨在评估通过尿道导管用咪达唑仑麻醉采集野生和圈养美洲虎精液的效率。六只圈养和五只野生美洲虎用咪达唑仑（0.08-0.1mg/kg）和氯胺酮（5mg/kg）联合进行化学保定。在咪达唑仑给药后，使用尿猫导管（直径 1mm×长 130mm）对尿道进行导管插入术，以从所有动物中收集精液。使用该技术，我们平均可以收集到含有 2,635.2 个精子/ml 的 347.2μl 精液。前向运动精子、精子运动能力和精子形态分析表明，该方法不会影响精子质量。因此，咪达唑仑给药后进行尿道导管插入术是一种从野生和圈养美洲虎采集高质量精液的实用且有效的方法；这将为美洲虎的繁殖辅助技术的发展铺平道路。
