Wildlife Research Laboratory, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, 78060-900, Brazil.
Veterinary Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, 78060-900, Brazil.
Theriogenology. 2020 Sep 1;153:34-38. doi: 10.1016/j.theriogenology.2020.04.035. Epub 2020 Apr 29.
The α-adrenergic agents are known to influence erection and ejaculation, and ejaculatory reflex is a primarily α-adrenergically mediated event. This paper aims to describe the urethral catheterization technique after pharmacological induction for semen collection in domestic dogs. For dose determination, semen collection was performed using increasing doses of dexmedetomidine (5 μg/kg, n = 2; 10 μg/kg, n = 2 and 15 μg/kg, n = 2) associated to 3 mg/kg ketamine in six animals. Once the dose was established, additional semen collection using association of 15 μg/kg dexmedetomidine and 3 mg/kg ketamine was carried out in eight dogs. After the procedure, sperm volume (0.092 ± 0.03 mL), total motility (58.33 ± 8.7%), vigor (1.89 ± 0.309), sperm concentration (1186.67 ± 304.667 × 10 sperm/mL), sperm plasma membrane integrity (83.22 ± 4.82%), acrosomal integrity (73 ± 6.187%) and normal sperm morphology (53.22 ± 5.6%) were assessed. Thus, this study demonstrates that urethral catheterization after pharmacological ejaculation using dexmedetomidine-ketamine association can be performed in dogs and it may be possible to obtain good semen quality, despite urine contamination.
α-肾上腺素能药物已知会影响勃起和射精，而射精反射主要是通过α-肾上腺素能介导的事件。本文旨在描述在药理学诱导后采集犬精液时的尿道插管技术。为了确定剂量，使用越来越多的右美托咪定（5μg/kg，n=2；10μg/kg，n=2 和 15μg/kg，n=2）联合 3mg/kg 氯胺酮对六只动物进行精液采集。确定剂量后，在另外八只狗中使用 15μg/kg 右美托咪定和 3mg/kg 氯胺酮联合进行额外的精液采集。在该程序之后，评估了精子体积（0.092±0.03mL）、总活力（58.33±8.7%）、活力（1.89±0.309）、精子浓度（1186.67±304.667×10 个精子/mL）、精子质膜完整性（83.22±4.82%）、顶体完整性（73±6.187%）和正常精子形态（53.22±5.6%）。因此，本研究表明，使用右美托咪定-氯胺酮联合进行药理学射精后可以在犬中进行尿道插管，尽管存在尿液污染，但仍有可能获得良好的精液质量。
