正畸矫治器早期释放的镍诱导细胞凋亡的重要途径——初步数据。
An important pathway of apoptotic effect of nickel early released from orthodontic appliances - Preliminary data.
机构信息
Department of Orthodontics, Medical University of Bialystok, Białystok, Poland.
Department of Pharmacodynamics, Medical University of Bialystok, Białystok, Poland.
出版信息
Pharmacol Rep. 2018 Aug;70(4):766-768. doi: 10.1016/j.pharep.2018.02.019. Epub 2018 Mar 21.
BACKGROUND
Limited data demonstrate the effect of nickel released from orthodontic appliances. The mechanism of this action is not clear. The present study aimed to investigate the role of kynurenines, oxidative stress and caspase pathway in the mechanism of nickel action.
METHODS
We studied the concentration of nickel, 3-hydroxykynurenine, total oxidative status in saliva and caspase-3 in epithelial cells in 10 subjects before and one week after orthodontic treatment.
RESULTS
Orthodontic appliances significantly enhanced the concentration of nickel, 3-hydroxykynurenine, total oxidative status and augmented the expression of caspase-3 seven days after treatment in the oral cavity in respect to pre-treatment values.
CONCLUSION
Our data suggest that nickel released from orthodontic appliances activate tryptophan metabolism in oral cavity via the kynurenine pathway. The metal directly or through kynurenines enhancement activates oxidative stress and then via the caspase pathway induce apoptosis of buccal epithelial cells.
背景
有限的数据表明了正畸矫治器释放的镍的作用。其作用机制尚不清楚。本研究旨在探讨犬尿氨酸、氧化应激和半胱天冬酶途径在镍作用机制中的作用。
方法
我们研究了 10 名受试者在正畸治疗前和治疗后一周唾液中镍、3-羟基犬尿氨酸、总氧化状态和上皮细胞中 caspase-3 的浓度。
结果
正畸矫治器显著提高了镍、3-羟基犬尿氨酸、总氧化状态的浓度，并在治疗后 7 天增加了口腔中 caspase-3 的表达，与治疗前相比。
结论
我们的数据表明，正畸矫治器释放的镍通过犬尿氨酸途径激活口腔中的色氨酸代谢。金属直接或通过增强犬尿氨酸激活氧化应激，然后通过半胱天冬酶途径诱导颊上皮细胞凋亡。
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