School of Science, Northeast Forest University, Harbin, Heilongjiang 150040, China.
School of Science, Northeast Forest University, Harbin, Heilongjiang 150040, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Aug 15;1092:343-349. doi: 10.1016/j.jchromb.2018.06.023. Epub 2018 Jun 21.
The plant hormone auxin plays a fundamental role in the growth and development of plants. However, because of its low concentration and many other interfering substances in plant tissues, the determination of auxins is still a challenging problem. To solve this problem, a kind of novel cellulose magnetic molecularly imprinted polymer micro-spheres (CMMIPs) was prepared by entrapment technique in this study. The FeO paramagnetic particles acted as the core and were modified by cellulose, silanized β-Cyclodextrin (β-CD) and 4-Vinylpyridine (4-VP), were adopted for the specific selective recognition cavities. The CMMIPs were characterized with scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). High performance liquid chromatography (HPLC) was used for the determination of IAA in plant tissues. Based on the evaluations, the results showed that the CMMIPs possess porous surface appearance, narrow range particle size distribution and fine roundness. The sizes of most CMMIPs were within the range of 50-180 μm. And they can extract IAA more efficiently than those cellulose magnetic non-imprinted polymer micro-spheres (CMNIPs). CMMIPs can be recycled and preserved for a long time. Effective linear ranges for IAA was in the range of 1.00-100.0 μg L, and the effective detection limits was 4.5 μg L. The adding standard recovery was about 95%, and the relative standard deviations (RSD) was <5% for standard sample. The performance analysis was also estimated by seeds from three different plants, pea, rice and wheat. Recoveries were ranging from 73.2-80.1%, and the RSDs were from 3.8% to 13.3%. The results show that it was a reliable analytical method based on CMMIPs extraction combined with HPLC.
植物激素生长素在植物的生长和发育中起着基本作用。然而，由于其在植物组织中的浓度低和许多其他干扰物质，生长素的测定仍然是一个具有挑战性的问题。为了解决这个问题，本研究采用包埋技术制备了一种新型纤维素磁性分子印迹聚合物微球（CMMIPs）。FeO 顺磁颗粒作为核心，并用纤维素、硅烷化 β-环糊精（β-CD）和 4-乙烯基吡啶（4-VP）进行修饰，作为特定的选择性识别腔。采用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对 CMMIPs 进行了表征。高效液相色谱（HPLC）用于测定植物组织中的 IAA。基于评价，结果表明，CMMIPs 具有多孔表面形貌、窄粒径分布和良好的圆度。大多数 CMMIPs 的粒径范围在 50-180 μm 之间。并且它们比纤维素磁性非印迹聚合物微球（CMNIPs）更有效地提取 IAA。CMMIPs 可以回收和长期保存。IAA 的有效线性范围在 1.00-100.0 μg L 之间，有效检测限为 4.5 μg L。添加标准回收率约为 95%，标准样品的相对标准偏差（RSD）<5%。还通过来自三种不同植物（豌豆、水稻和小麦）的种子对性能分析进行了评估。回收率在 73.2-80.1%之间，RSD 在 3.8%-13.3%之间。结果表明，基于 CMMIPs 提取与 HPLC 结合是一种可靠的分析方法。
