一种基于表面增强拉曼散射的方法，用于快速、高灵敏度定量分析慢性肾病患者的生理盐水、人血清和尿毒症血清中的3-羧基-4-甲基-5-丙基-2-呋喃丙酸酯和吲哚-3-乙酸。

A surface-enhanced Raman scattering-based approach for rapid and highly sensitive quantitative analysis of 3-carboxy-4-methyl-5-propyl-2-furanpropionate and indole-3-acetic acid in saline, human serum and uremic serum of patients with chronic kidney disease.

作者信息

Saadati Shaghayegh, Eduok Ubong, Abdelrasoul Amira, Shoker Ahmed

机构信息

Department of Chemical and Biological Engineering, University of Saskatchewan 57 Campus Drive Saskatoon Saskatchewan S7N 5A9 Canada

Division of Biomedical Engineering, University of Saskatchewan 57 Campus Drive Saskatoon Saskatchewan S7N 5A9 Canada.

出版信息

RSC Adv. 2020 Dec 8;10(71):43489-43496. doi: 10.1039/d0ra06123a. eCollection 2020 Nov 27.

3-Carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and indole-3-acetic acid (IAA) are critical protein-bound uremic toxins that occur during chronic kidney disease (CKD). This study offers the first reported instance of surface-enhanced Raman scattering (SERS) coupled with an Au nanoparticle substrate for the simple quantification of CMPF and IAA in human serum samples. The detection limits of the CMPF and IAA analysis were estimated to be 0.04 nM (S/N = 3) and 0.05 μM (S/N = 3), respectively. The results demonstrate that the SERS technique is fast-acting and highly sensitive when it comes to the simultaneous and individual quantitative detection of CMPF and IAA in biological samples. We believe that this analytical tool could serve as a very useful method for practical applications during the analysis of CMPF and IAA in the serum and urine of patients at all stages of CKD and of healthy volunteers as well as in various reservoirs.

3-羧基-4-甲基-5-丙基-2-呋喃丙酸酯（CMPF）和吲哚-3-乙酸（IAA）是慢性肾脏病（CKD）期间出现的关键蛋白结合尿毒症毒素。本研究首次报道了表面增强拉曼散射（SERS）与金纳米颗粒基底相结合，用于简单定量人血清样本中CMPF和IAA的实例。CMPF和IAA分析的检测限估计分别为0.04 nM（S/N = 3）和0.05 μM（S/N = 3）。结果表明，SERS技术在生物样品中同时和单独定量检测CMPF和IAA时具有快速起效和高灵敏度的特点。我们相信，这种分析工具对于在CKD各阶段患者以及健康志愿者的血清和尿液中以及各种样本库中分析CMPF和IAA的实际应用而言，可能是一种非常有用的方法。