Cimpean Anca Maria, Lalošević Dusan, Lalošević Vesna, Banović Pavle, Raica Marius, Mederle Ovidiu Alexandru
Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
Department of Histology and Embryology, Medical Faculty, University of Novi Sad, Novi Sad, Serbia.
In Vivo. 2018 Jul-Aug;32(4):791-798. doi: 10.21873/invivo.11309.
To characterize baby hamster kidney fibroblast (BHK 21/C13) cells and test the effects of antibodies against podoplanin and disodium cromolyn on BHK 21/C13 cell line-derived tumors grown on chick embryo chorioallantoic membrane (CAM).
BHK 21/C13 cell-derived fibrosarcomas developed in hamsters were implanted on CAM and treated with anti-podoplanin antibodies and disodium cromolyn. BHK 21/C13 cell immunophenotype was assessed.
Fibrosarcoma cells were positive for vimentin, CD117, smooth muscle actin, vascular endothelial growth factor epidermal growth factor receptor, homebox prospero gene 1 and negative for platelet-derived growth factor B, neuron-specific enolase, S100, CD34, Ewing sarcoma and podoplanin. CAM-grown fibrosarcomas were highly sensitive to disodium cromolyn and anti-podoplanin antibodies.
Immunophenotyping BHK 21/C13 cells and their response to drugs represent the first step in revealing cell line utility and a reliable tool for experimental cancer research.
鉴定叙利亚仓鼠肾成纤维细胞（BHK 21/C13），并测试抗血小板内皮细胞黏附分子抗体和色甘酸钠对在鸡胚绒毛尿囊膜（CAM）上生长的源自BHK 21/C13细胞系的肿瘤的影响。
将在仓鼠体内形成的源自BHK 21/C13细胞的纤维肉瘤接种于CAM上，并用抗血小板内皮细胞黏附分子抗体和色甘酸钠进行处理。评估BHK 21/C13细胞的免疫表型。
纤维肉瘤细胞波形蛋白、CD117、平滑肌肌动蛋白、血管内皮生长因子、表皮生长因子受体、同源盒prospero基因1呈阳性，而血小板衍生生长因子B、神经元特异性烯醇化酶、S100、CD34、尤因肉瘤和血小板内皮细胞黏附分子呈阴性。在CAM上生长的纤维肉瘤对色甘酸钠和抗血小板内皮细胞黏附分子抗体高度敏感。
对BHK 21/C13细胞进行免疫表型分析及其对药物的反应是揭示细胞系效用的第一步，也是实验性癌症研究的可靠工具。
