Gören-Sağlam Nihal, Harrison Elizabeth, Breeze Emily, Öz Gül, Buchanan-Wollaston Vicky
1Division of Botany, Biology Department, Faculty of Science, Istanbul University, Istanbul, Turkey.
2School of Life Sciences, University of Warwick, Coventry, CV4 7AL UK.
Physiol Mol Biol Plants. 2020 Apr;26(4):733-745. doi: 10.1007/s12298-019-00752-7. Epub 2020 Feb 6.
Leaf senescence is an important developmental process for the plant life cycle. It is controlled by endogenous and environmental factors and can be positively or negatively affected by plant growth regulators. It is characterised by major and significant changes in the patterns of gene expression. Auxin, especially indole-3-acetic acid (IAA), is a plant growth hormone that affects plant growth and development. The effect of IAA on leaf senescence is still unclear. In this study, we performed microarray analysis to investigate the role of IAA on gene expression during senescence in . We sprayed IAA on plants at 3 different time points (27, 31 or 35 days after sowing). Following spraying, PSII activity of the eighth leaf was evaluated daily by measurement of chlorophyll fluorescence parameters. Our results show that PSII activity decreased following IAA application and the IAA treatment triggered different gene expression responses in leaves of different ages.
叶片衰老对于植物生命周期而言是一个重要的发育过程。它受内源性和环境因素控制，并且会受到植物生长调节剂的正向或负向影响。其特征是基因表达模式发生重大且显著的变化。生长素，尤其是吲哚 - 3 - 乙酸（IAA），是一种影响植物生长和发育的植物生长激素。IAA对叶片衰老的影响仍不清楚。在本研究中，我们进行了微阵列分析，以研究IAA在衰老过程中对基因表达的作用。我们在3个不同时间点（播种后27、31或35天）向植物喷洒IAA。喷洒后，通过测量叶绿素荧光参数每天评估第八片叶子的PSII活性。我们的结果表明，施用IAA后PSII活性降低，并且IAA处理在不同年龄的叶片中引发了不同的基因表达反应。
