Kolodziej Marcin, Melgies Alicja, Joniec-Wiechetek Justyna, Michalski Aleksander, Nowakowska Anna, Pitucha Grzegorz, Niemcewicz MarcinN
Biological Threats Identification and Countermeasure Centre, Military Institute of Hygiene and Epidemiology, Pulawy, Poland.
Provincial Sanitary Epidemiological Station, Rzeszow, Poland.
Ann Agric Environ Med. 2018 Jun 20;25(2):368-373. doi: 10.26444/aaem/90535. Epub 2018 May 22.
Dobrava-Belgrade virus (DOBV) is one of the emerging pathogens which have been reported during the last decades in Europe and have attracted the attention of researchers. The course of infection among humans may have a varied course - from the completely asymptomatic to the more severe forms, such as haemorrhagic fever with renal syndrome (HFRS). DOBV is hosted and carried by rodents like Apodemus flavicollis or A. agrarius, which occur commonly in Europe.
To-date, orthohantaviruses have been reported in Poland, both in humans and animals, but detailed country-scale studies have not yet been carried out. The aim of the study was molecular characterization of a strain which was found in A. flavicollis in south-eastern Poland.
The phylogenetic analysis of the first Dobrava-Belgrade virus found in A. flavicollis in the subcarpathian region of south-eastern Poland, presented in this study, was performed after virus proliferation in cell culture and sequencing of specific PCR products.
Based on genetic sequences of fragments of three segments (S, M and L), the isolated virus was assigned to the Dobrava genotype, taking into consideration the most current classification of the DOBV species.
The Dobrava-Belgrade virus strain isolated from A. flavicollis in the subcarpathian region of south-eastern Poland, has been molecularly characterized and assigned to Dobrava genotype, thereby the occurrence of that genotype in Poland has been confirmed by molecular techniques.
多布拉伐-贝尔格莱德病毒（DOBV）是过去几十年在欧洲出现的病原体之一，已引起研究人员的关注。人类感染该病毒后的病程可能各不相同——从完全无症状到更严重的形式，如肾综合征出血热（HFRS）。DOBV由黄颈姬鼠或黑线姬鼠等啮齿动物携带，这些动物在欧洲很常见。
迄今为止，波兰已报告在人类和动物中均发现汉坦病毒，但尚未开展详细的全国范围研究。本研究的目的是对在波兰东南部发现的一株黄颈姬鼠携带的病毒进行分子特征分析。
本研究对在波兰东南部喀尔巴阡山地区黄颈姬鼠中首次发现的多布拉伐-贝尔格莱德病毒进行了系统发育分析，该分析是在病毒在细胞培养中增殖并对特定PCR产物进行测序后进行的。
根据三个片段（S、M和L）的遗传序列，考虑到DOBV物种的最新分类，将分离出的病毒归为多布拉伐基因型。
从波兰东南部喀尔巴阡山地区黄颈姬鼠中分离出的多布拉伐-贝尔格莱德病毒株已进行分子特征分析并归为多布拉伐基因型，从而通过分子技术证实了该基因型在波兰的存在。
